The following images are from ARPE-19 Cells cultured for 3 Weeks over here in Nottingham (thanks for help from Emilia Moradi, Victoria Ciampani, and Kevin Webb). MgF2 nanomembrane chips were positioned into a transwell insert and sealed using PDMS, a biocompatible polymer, creating a two-compartment transwell. After an ethanol sterilization, ARPE-19 cells were seeded in a 12 well…
Overview – The purpose of this study was to perform in vitro hemocompatibility testing (in accordance with ISO standards) on material substrates that could come in contact with blood when incorporated into the final device design of a portable hemodialysis machine. Therefore, understanding the blood response to the lift-off membrane (NPN with SU-8 grid, supported…
Introduction: At the last meeting, I introduced the new stirred cell device acquired in Tom’s lab (here). I have expanded the study to observe changes in flux and concentration for each of the membranes used. Experiments: Each of the experiments was run with BSA having an initial concentration of 1mg/mL, this concentration is determined using spectrophotometry…
I assembled two 2-chip dialyzers using the thicker, 75-nm NPN membrane chips (10 mm long multichannel, 22 mm x 24 mm). The dialyzers were pumped full of IPA, to fill with fewer bubbles, then infused with PBS for at least one hour. Unfortunately both of these either broke when hooked up to the animal, or…
In my earlier posts, as well as in Josh’s NPN paper and Tom’s ACS nano paper, we’ve talked a lot about the resolutions of nanoparticle separations. What we mean by it is how well the membrane is able to discriminate between two similarly sized particles. For instance, we predict that a series of gold nanoparticle filtrations…
Introduction: The lab purchased an Amicon 8010 Stirred Cell which has a 25mm diameter for membranes and holds 10mL of solution. The device works by placing the solution into the cell, pressurizing the system at 8-10 psi, and creates stirring via an elevated stir bar and the stir platform. This type of filtration system was chosen…
Since the new year there have been difficulties with charging the glass plate and the charge not being enough to capture the membrane. Synthetic silk was tested to see if the glass charged better but no difference was seen between the silk and a crew wipe. When materials were first being tested to use during…
Objective Develop an easily mainpulated reproducible vasculogenesis assay using hydrogels laden with endothelial cells (‘bio-ink’) patterned using the technique of extrusion bioprinting Bio-inks Develop a bio-ink with the following characteristics Cell viable: bio-ink must sustain cell life, allowing cells to manipulate and move through the hydrogel Vasculogenic: bio-ink must encourage (or at least not negatively affect) tubule formation…
As part of my work over at the University of Nottingham, I’ve been using iBidi devices to grow cells. Eventually, I will use their product to do Raman studies of cells grown on my MgF2 substrates. I’ve been making some progress towards that end using PDMS (I’ve been using 10:1 base to curing agent for…
Hey all! In this post, I’d like to present some of my final data on the exosome isolation project and cover some data that was generated in response to questions asked during our previous discussion. To begin with, Tom asked whether or not it was possible to measure protein in the filtrate and retentate and…