I recently got some good looking results on the FLAG diffusion test over a 24 hour period. The relative concentrations of enzyme seemed to indicate that measurably more enzyme diffused from retentate to filtrate in solutions not containing constricting Anti-FLAG antibody, and this is excellent news, but over the 24 hours between beginning the diffusion…
We’ve been wondering for some time just how the thinness of our membrane will affect the concentration polarization behavior of solutes. Because solutes with sieving coefficients less than 1 are (by definition) moving slower than the solvent around them, in the area before the membrane their concentration increases. In the case of proteins, this so-called…
Helpful Hint: H<sub>2</sub>O typed as text will yield H2O visually, and E = mc<sup>2</sup>O typed as text will yield E = mc2. Of course this affects line height, so choose your poison, I guess.
I’ve been on a Sepcon assembly binge, and I thought I would post my assembly statistics. On 12/15/13 (assembly “L”) I broke 28 chips out of wafer 1070. 3 chips were visually damaged by the break-out process, 1 was already defective. After inserting the chips into the sepcon housing, testing with 5 psi nitrogen revealed…
Earlier this year an MIT/Harvard group generated and transplanted a rat kidney and published in Nature Medicine Technical Report. “Regeneration and experimental orthotopic transplantation of a bioengineered kidney” Jeremy J Song, Jacques P Guyette, Sarah E Gilpin, Gabriel Gonzalez, Joseph P Vacanti, & Harald C Ott Center for Regenerative Medicine, Massachusetts General Hospital and Harvard…
Images of the nanoporous nitride wafer. The same wafer that Josh declared as having 30% porosity with an average pore size of 80 nm (or something similar). Josh put images and such on basecamp for those who have access. These numbers are for a TEM grid from near the outside edge of the wafer, where…
The Wiesner lab at Cornell (whom you may remember as the discoverer of the neutrally charged but highly fluorescent “C-dots” Jess used for her charged-based separation work) are about to publish Asymmetric Organic-Inorganic Hybrid Membrane Formation via Block Copolymer-Nanoparticle Co-Assembly in Nano letters. Building upon their previous work on block copolymer self-assembling films, they have added titanium dioxide nanoparticles…
Goal: Measure SU-8 shrinkage that have gone through various soft and hard bake conditions To measure the shrinkage, the SU-8 scaffolds needed to be lifted off, but there was some issues. Originally, the SU-8 was spincoated onto an oxide layer, which would be etched away using BOE. The SU-8 had appeared to be disintegrating due…
I tested all the microbeads that the Gaborski and Lapizco-Encinas labs own. The results are summarized below. The sizes ranged from 50 nm to 2 µm. I mixed them to concentrations of 0.2 g/L for all bead less than 1 µm and 1.0 g/L for all 1 µm or greater. Interestingly, the scans look rather…
From last few months, I am working on the development of a co-culturing system that can work as an in vitro mimetic of the human vasculature. The idea is to make a device capable of co-culturing, real time imaging, equipped with fluidics for testing different assays as well as to grow cells under shear. TEER measurement…