In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two pnc-Si chips mounted in teflon block with blood flowing between the chips and dialysate flowing…
This is an update to my last post, where HUVECs mixed with collagen inside PDMS channels seemed to stay rounded up and not really do much of anything exciting. This time around I made a few adjustments: New collagen protocol – gives 1.5 mg/mL solution before mixing with cells: -10% 10x PBS -1.4% NaOH -38.6%…
In a previous post I had shown the relative fluorescence of various substrates as normalized to glass. 1um beads (either green or red) were placed onto each surface and imaged with constant EM Gain, exposure time, and intensity. Bar graphs were constructed to show the relative fluorescence (red and green were not normalized to each other)….
In this previous post (also some in this post), I had displayed the difference between the 120nm SiO2 microporous membranes and four different iterations of Track-Etched insert membranes in reference to nuclear (DAPI, blue) and actin (Phalloidin, green) stain. I recently performed the same staining protocol on other substrates: High Pore Density Microporous 300nm SiO2, Low…
I’ve redesigned the co-culture devices described in an earlier post to allow for media isolation as well as to alleviate concerns regarding bubble trapping. The new design is pictured below: The large cube of silgard builds a cylindrical reservoir for media and can support upwards of 100 uL of fluid (equivalent to other co-culture devices…
After last week’s post detailing the problems I’ve been having with my streaming potential setup, Jirachai was kind enough to come by the U of R last friday to look at my device. We banged our heads into the wall for a good two hours, but the drifting potentials and apparent insensitivity of the streaming…
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic
This is a cursory review of the nonotechnology articles appearing along with our paper in the Journal “Advances in Chronic Kidney Disease” Most of these are reviews and most deal with nanoparticles for either imaging or therapy. One does discuss nanoparticles for use with membranes to improve biocompatibility. I do not have full access to…
Scope: Human umbilical vein endothelial cells (HUVEC) and adipose derived stem cells (ADSC) were co-cultured on SiO2 low density 3um pore membranes. The ADSCs were initially seeded onto the membranes, at 6 hours the membranes were flipped over and placed into a 24 well plate and HUVECs were seeded. Cells were grown for 3 days…
Blanca, Tom, Aytug and I are working on a proposal to NSF that will be submitted Oct.29th to the Chem. & Bio. Separations Program of the CBET division. This is a joint 3-year project between Blanca’s and Tom’s labs. Basically, we are combining Blanca’s insultator-dielectrophoresis with the microporous version of the SiO2 (or SiN) ultrathin…