NRG

Here’s a few sketchup animations I made for Dean’s device, based on his PPG presentation last week:      

Poor English Paper used as a starting point: Silicon Nitride Etch Rate paper This is a continuation of the work I’ve done, attempting to make freestanding MgF2 nanomembranes (theory and deposition) using a Silicon Nitride nanoporous substrate. I originally  wanted to use hot phosphoric acid to remove the Si3N4, but since we do not have that…

In this experiment, I soaked membranes (from either wafer 1064 [150W deposition power] or wafer 1065 [100W deposition power]) in different fluids that are standard use in cell culture applications, or which provide a similar situation as could be found in such (cause of pH change, salts, etc.). Each membrane is made into a single-well…

EDIT – 2/10/14 – Adding 1 mM phosphate buffer fixes the problems outlined in this post. I’ve been having some trouble taking consistent pH measurements with the new pH probe. Prior to Thanksgiving I made a spectrum of 10 mM KCl buffers (at 4, 6, 7, 8, and 10 pH units) for use in my…

In evaluating the usefulness of our membranes for cell culture, it is important to understand if and how cell growth is affected by the surface.  The desire is that they be consistent with standard culture substrates (i.e. Tissue Culture Plastic (TC)). I performed two growth trials using HUVECs (Human Umbilical Vein Endothelial Cells), one focusing…

I am attempting to fabricate a free standing, porous, MgF2 nanomembrane for Raman spectroscopy applications (see my previous post for background). To that end, I’ve used the Lesker p75 evaporator in the basement to deposit ~100nm of MgF2 on one of our nanonitride Sepcon chips (Wafer 1055, 50 nm SiN, 2% porosity, 20-40 nm pores)….

I recently popped out membranes from wafers 1064 and 1065 for our lab. I only started with about half a wafer, but these are the numbers from the extraction: Wafer ID # of In-Tact Extracted Membranes : High/Low Porosity # I broke during extraction # already broken before extraction # apparently under etched 1065 (100W)…

For context, see my previous post. Early on in the process of designing an experiment to confirm the usefulness of a CytoVu assembly for use as an assay, it was decided that the best solution for measuring the extremely low concentrations [ng/mL scale] of bacterial alkaline phosphatase (“bAP”) in the filtrate which would indicate what…

My streaming potential device is now working consistently. It turns out that the problem was the concentrations of salt that I was using. The Debye Length (the distance over which charge-charge interactions are important in an electrolyte) is given as , where   is the debye length in nm and  is the molar concentration of…

In working on a long over due paper by Barrett on endothelial response to high permeability material … Key figs … Figure 2 Figure 4 Figure 5 And one post from Nakul. I came across this highly relevant paper Pedrigi(Overby)EER11