NRG

In my last post I reported findings that the bottom well of a CytoVu assembly is capable of adsorbing protein at about 0.03 ug/mL, or 0.75ng of protein. This is a fairly small value, but for the purposes of a sensitive separation such as the FLAG tests that I’ve been working on, it can be…

Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films ABSTRACT: Despite considerable efforts toward fabricating ordered, water-permeable, mesoporous films from block copo- lymers, fine control over pore dimensions, structural character- istics, and mechanical behavior of graded structures remains a major challenge. To this end, we describe the fabrication and performance characteristics of…

Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells  and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used as matrices for the cells, the bottom of the plate was used as the control….

After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’ red 1µm beads (in 1X PBS) onto each type of membrane (the SiO2 and SiN…

In the wake of the apparent loss of all of the phosphatase in a sample that was placed into a CytoVu well, left for 24 hours, and then checked for phosphatase activity, it’s become clear that the adsorptive qualities of the CytoVu assemblies needs further characterization. I showed in an earlier post that there was some loss…

So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made some household cell culturing platforms as shown in the figure below. filled the whole petri…

EO tests were continued using 2 micron carboxylated PS Latex beads in a 0.2 mM K2HPO4 solution containing 0.1% tween. The solution had a pH of 7.54 and a conductivity of 67.0 uS/cm. The beads were negatively charged, and so would move towards the positive electrode absent electroosmotic flow. It was suggested that the pump…

This is a follow up to Josh’s post, “Continued Au Separations with SiN-NP wafer #’s 1029 and 1030”, and seems to explain why we initially got an S value greater than one during our gold separations. We conducted tests to see if the colored centrifuge tubes were at fault for our seemingly large filtrate absorbance…

As I mentioned in my previous post, there are a lot of problems associated with the technique for testing the ability of a CytoVu assembly to act as an assay that I had been trying to use before. Several possible sources of error were apparent, such as the difficulty inherent in consistently pipetting such small…