The initial measurements with multiple tests tubes containing either Serum or PBS failed to demonstrate evaporation and in fact the weights of the collection tubes increased after sitting in the refrigerator overnight. Perhaps the tape collected water? At any rate (<– trite expression), I redid the experiment with a single tube of serum. The tube…
Two experiments were performed to explore application of SiMPore filters within INT’s microfluidic cartridges to allow the capability for pathogen type specificity in nucleic acid purification. 1) The first experiment tested the efficiency at which nucleic acids could pass through the membranes. T4 polynucleotide kinase was used to 32-P radiolabel two different sized PCR products:…
Summary of Results Membrane Pore Diameter Thickness Porosity Permeability (cc/cm^2*min*bar) SiN 3.2-4.0 um 0.12 um 22.1%-35.3% 30000 PCTE 2.4-3.0 um 9 um 11.9%-16.1% 1652 SiN Experimental Design Permeability of the 3 um pore Si Nitride membranes was assessed using the pictured apparatus: Fluid (ultrapure water) was pumped at a constant rate into a pipet whereupon…
After discussions with the gentleman in charge of surface functionalization at INT last week, Jim suggested that I attempt to pretreat our membranes with 0.1% SDS before a filtration. The hope being that some surfactant left on the surface of the membrane would be enough to facilitate water passage through the membrane without wetting the…
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This summer I’ll be working on preliminary development of technology that might lead to SiMPore drug discovery assays. The goal is to use SiMPore membranes to create much more efficient and generalized assays, which represents a big opportunity for the company. However, several challenges need to be addressed in order to make such a device…
Can someone help explain this e-mail? Hey Jim – Just got a call from the locksmith who had a report from security that someone in your lab is tampering with the lock on your lab door…by doing what they’re doing, they are able to get into your lab without a key (or swiping). It’s been…
This is the setup used for the long term clearance testing. The fraction collector can be set to advance to the next collection tube at set intervals from 1 min to 99 min. I am currently conducting an evaporation test with both PBS and 100% serum. I weighed six collection tubes, two were filled with…
A new user in URnano has used the “old” susceptor for a ~270 C (I might have the exact number wrong, but it is relatively low temp) to heat In on GaAs. I don’t think there was ever a good reason for using the RTP for this process, but what is done is done. I…
Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and DI or PBS. We are testing for what will make the most optimal cell culture…