Here is an aggregate of the recent posts/data on the Hemodialysis Project … Device Design/Demo Design idea for assembling multiple chips Gasket design for counter flow dialysis chip Peclet chip demo – > Dialysis Membrane modifications to prevent protein binding (HD PEG) Protein binding is reduced by both Adarza and Shestopolov treatments Damage of pnc-Si by…

Hi all, A while back (in the link below) I have posted the analytical solution that allows us to solve the flow rate distribution in our shear-free system. shear stress reduction via pnc-Si: analytical solution However we do not have a closed-form solution at that time. After a brief discussion we believe that there should…

If you know me at all, you know I enjoy nothing more than ultrafiltration and Parylene-C deposition. Now these activities are combined into one device to improve the biocompatibility (platelet activation and adhesion) of nanoporous membranes. In this letter, polyethersulfone (PES) membranes were coated with Parylene-C, poly(chloro-para-xylylene) aka (PCPX), while glycerin vapors rising through the…

We deposited a 20/60/20 nm OSO stack for an ellipsometry test.  We decided to try annealing the un-patterned wafer in the RTP at 1050 C with no susceptor with a 100 C/s ramp for 60 s. The attached SEM images show pores forming.  With these encouraging results, we are considering depositing 40, 50 and 60…

In collaboration with Alex Shestopalov’s lab, I started working on liquid based PEG treatment on windowed sepcon chips. The treatment is a 3-day long protocol and involves multiple washing and drying steps with different reagents. Most of our sepcon chips broke down in first few steps itself. The initial wash consists of Nanostrip (sulfuric plus nitric…

  These Adarza chips were pre wetted with PBS then incubated with fetal bovine serum (FBS) overnight in the refrigerator then rinsed. TRITC (2 mg/mL) added to  chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI) water. I first measured using the…

Josh and I are taking a closer look at how the samples burst and what different factors can affect the strength of the membrane. We are specifically looking at minimum dimension, area, and perimeter. A wafer was designed that contained different sized samples. One of the three factors were held constant while the other two…

In a previous post, we showed that both Cytochrome C and BSA at concentrations of ~1mg/mL through 20nm cutoff Sepcons with about the same filtrate concentration (~60% of the feed concentration). Yesterday (1/14/13) we repeated these experiments with 10 and 15nm cutoff Sepcons and demonstrated pretty clearly that either 10 or 15nm Sepcons can be used…