This is a summary of diffusion experiments with A3G that were conducted during the week of 5/10/2010-5/14/2010. The HMM structure of A3G is a large protein multimer held together with RNA. Since the ultimate goal of this project is to develop a screening assay for HIV drugs, a successful experiment is one that will show…
Hi Guys, I thought this might be interesting, especially with the interest in SiN filters. While playing around with the SiN membranes for my project over in ChemE, I fought that the 1 micron SiN is surprisingly strong and that I can place our silicone gaskets onto the membrane and remove it without damage. By placing the…
With the goniometer nearly finished, I’d like to share a look at what has been created: Introduction A surface contact angle goniometer (SCAG) measures the angle(s) of contact that a droplet of liquid makes with a surface. This particular SCAG does this by creating a shadow that is isometric with the droplet and recording its…
Before the fresh Cytochrome C arrived, I ran two samples. The first sample was our old Cytochrome C in 0.3mM sodium phosphate buffer at a concentration of ~1mg/mL. I passed it through the 20nm cutoff 3rd generation Sepcons by spinning it in the small centrifuge at 2000rpm for 5 minutes. Approximately 100uL of sample passed through…
A group published a paper in Lab on a Chip (2012) using track-etched membranes for electro-osmosis. There are a lot of eerily familiar terms and concepts in the paper. The title even sounds familiar: Low-voltage electroosmotic pumps fabricated from track-etched polymer membranes They created a device with metal mesh electrodes on the opposite sides of…
Josh has been kind enough to fabricate a shadow mask for me to create interdigitated electrodes without the hassle of photolithography and etching. In order to be able to use this method, I want to make sure the produced electrodes are the same or better than the old method. I worry about shadowing effects due…
pnc-Si chips prewetted with PBS then incubated with fetal bovine serum (unknown molarity) for 2 hours under refrigerated conditions. Thoroughly rinsed with reaction buffer (perfused 5 times). TRITC (2 mg/mL) added to dry chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI)…
Recall that we’ve been having a great deal of trouble following the free ligand during our separations of the Bioconjugated Barcikowski particles, and that this trouble also means we can’t see the ligand after centrifuging the pure sample. In the interest of thoroughness, I replicated the assay the Barcikowski lab used to quantify the unbound…
In order to verify the validity of the result of the five-layer structure I did last time, I deposited another wafer with NOSON stack, which has the identical thickness as that of the last time. The annealing condition is also same as the last time, which is 1 minute holding time at 1000C with a…
During my visit last, I deposited a couple of wafers and one of them is to test a even thicker Si film from the NSN stack. The thickness for the Si film is 200nm and the nitride layers are still 30nm. After deposition, the Si wafer was etched and the pop-out TEM grids were then…