Karl, Charles and I measured the burst pressure and permeance of some 30-50-30 and 30-100-30 NSN. Burst Pressure: The SEPCONs readily burst at pressures under 1 psi when the flow was directed from the backside of the chip through the membrane. The membranes were able to withstand higher pressures when the pressure was applied from…
For this experiment, 3um diameter pores were created using SimPore’s mask. Reactive Ion Etching was used to form the pores in the SiN. Both 25nm and 150nm TEOS oxides were used. An SU8 Structure of 10um lines with 100um square windows was chosen. A sample was placed in 10:1 BOE w/ surfactant for various amounts…
On Friday(which was a great day for science). I ran a series of 5 passes of the bioconjugated gold nanoparticles through 30nm cutoff Gen 3 Sepcons. If you’ll remember, the Barcikowski lab wants the free ligand separated out from the complexes. To indirectly get the relative concentrations of gold and ligand, we measured the absorbance…
Nanoparticles Inventory – taken by Karl on 11/2/12. All these particles can be found in the refrigerator. Manufacturer size type amount (approx) expiration date fluorescence (if applicable) BBI 5nm gold colloid 3mL Jan-10 BBI 5nm gold colloid 6mL Aug-11 BBI 10nm gold colloid 2mL Jun-11 BBI 10nm gold colloid 2mL May-09 BBI 15nm gold colloid…
In this post, two unique five-layer structures will be discussed. The first five-layer structure is nitride/oxide/silicon/oxide/nitride (NOSON) with the deposition order from bottom to top. In this structure, the silicon film is 25nm while the nitride and oxide are both 15nm. After etching the substrate, the membrane was RTP annealed at 1000C for 1 minute….
In this post, we’ll discuss a little bit about how would the deposition temperature affect the pore formation and pore morphology. I first show some previous results of the effect of deposition temperature on the porosity and pore size from Dave. From this plot, it shows that the relative low deposition temperature barely affects the…
Katia in Dr. Shestopalov’s Lab functionalized some pnc-Si windowless chips. The process she follows is shown in Figure 1. She provided chips at various stages of the process. “Grignard”, “Diazirine”, and “PEG”. After functionalization, the chips were wetted with 20 µL of 1:1 pbs then 40 µL of 5 mg/mL f-BSA was added. The…
Etch Rate in BOE 10:1 Thermal Oxide TEOS PECVD Oxide LTO Sputtered 10:1 BOE at 20C 66 nm/min 266.4 nm/min > 180 nm/min * Each oxide type was placed in BOE w/ surfactant for 10 seconds, then the rate per minute was determined. *after 10 seconds no oxide remained on LTO sample therefore the exact…
Given the results of my previous post, I am reworking my design to make it more manufacturable. BBB Device V2-6 Compressed The major change compared to my last design is the inclusion of a full length microscope slide and coverslip. Instead of cleaving the electrodes from the glass, this step eliminates the need to…
To prepare the BBB devices for cell culture, they must be sterilized. To that end, I wanted to see if there were any adverse effects from autoclaving the device. Recipe 4 on the U of R autoclave is a dry process that we use to sterilize tips, so I used the same recipe. I placed…