After diffusing through a polymeric or pnc-Si membrane in the transwell format, diffusing molecules meet an “unstirrred water layer = UWL” on the bottom of the membrane. The UWL acts as another resistance to transport into the receiver compartment. According to some PAMPA papers, the UWL can be greater than 1 mm in thickness without…
This was mentioned in the meeting today, and I’ll try to describe what’s going on here. We start out with a system with a smaller volume on the top of the membrane: If we start out with 10 mg/mL in the retentate, and assume perfect equilibrium of the protein/particle: The concentrations in each compartment would…
This experiment is to determine if the time between the SC1 clean and when the wafers get deposited matter. Currently there is suspicion that an SC1 from an RCA clean should at RIT should be done right before deposition, . So what is done typically is that lithography steps are done weekly at RIT to…
I ran new experiments over the weekend in which I tested NaCl at 10, 25, 60, and 155mM. I also ran filtrations in the presence of 10mM LiCl and 10mM CsCl. These experiments were performed with chips from wafer # 024. I am only providing the Filtrate / Retentate ratios. It is obvious that the…
I tried particle separations with the 30kD cellulose membranes from Millipore using the same setup as previous experiments. Basically the results are that neither 5 nor 20nm particles pass through the membranes whether in water or 10mM KCl. Absorbance Data: Sizing Data: No peaks were found in the nm range for any of the filtrates….
A couple of weeks ago, I posted a discoloration array experiment that showed monotonic discoloration patterns here. This was a result that we’d been expecting for several months, but we never got it with NaCl and NaHCO3. Simplifying the solutions to NaCl and NaOH was the key. These results spurred some discussion on the mechanism…
A few weeks ago, we made wafers with three different silicon thicknesses: 15 nm, 30 nm, and 50 nm. The goal was to see how pore morphology changes with increasing thickness. The films were deposited at 450 C with a 25 W substrate bias. The annealing temperature was 800 C in the susceptor. Note that…
Here’s a cropped image from a recent TEM pic that Brian McIntyre took of our material for SiMPore (15 nm thickness, not sure what wafer). This particular feature caught my eye because the crystal is perfectly aligned so we are looking at individual atomic columns of Si, instead of the typical crystal planes. These happen…
The older sepcon macro has been update to fix previous issues and yield more meaningful results. These updates are being brought up to get feedback from the group and from there implement them and make these changes to the newer sepcon patterns and eventually the TEM patterns. Gathering and analyzing statistical data is standard practice…
As mentioned previously, gold colloids are not stable in high salts because salts reduce the repulsive forces between particles and cause aggregation. I have been able to dissolve naked gold colloids in 10mM KCl without aggregation, but the particles crash out at 100mM KCl. There are a couple of strategies that can be used to…