Summary My analysis of npMgF2 has relied heavily on both tomogram and segmentation processes. The tomogram generation has thus far been sufficient, giving enough detail to our eye to identify different structures, but turning the tomograms into 3d models has been a lot more labor intensive. Using a neural network classifier (Weka, out of a…
Introduction/Motivation Endothelial cell adherence mechanisms are well characterized in literature to date. Endothelial cells, such as HUVECs, interact with their substrate as well as surrounding cells through focal adhesions (network of proteins involved in cell adhesion; vinculin, talin, FAK, etc.), VE-cadherins, tight junctions, and other membrane bound molecules. These molecules work in combination to allow…
I created a COMSOL model (zip) model with geometry based on the CAD drawing of the sepcon fixture Jared provided. I’ve included Velocity results (then Concentration, Pressure, Vorticity, and Shear rate). Also included is the Parameter table which has the Diffusion Coefficient for Urea taking into account the drawn thickness of the membrane (100 µm) vs…
I am developing a process for fabricating microporous Parylene Liftoff membranes. I am interested in these as a more flexible substrate for cell culturing. Parylene is relatively strong (compared to say pdms), so it is feasible to work with lifted off pieces of membranes on the order of 1 micron or less in thickness. I list…
Intro Understanding how the Au film coats the inner part of the npMgF2 is essential to understanding how the photonic properties of the npAu/npMgF2 sensor arise. From SEM, we are able to resolve little cones of Au, but unable to see how rough/homogeneous/open the Au layer is. Our hypothesis is that these little cones of…
Many thanks to JK, Dean and Gunner (Webster HS) for compiling this table. Take homes … NPN-O has smaller pore sizes, lower porosity than NPN at the same thickness (50 nm). All materials have slightly asymmetric pores (~ R1/R2 = 0.75). Top-side and bottom side pore distributions are not the same. The original NPN material…
Within the past few weeks we have been working on finalizing an architecture for PETL dialysis that can be cut out, assembled, and disassembled repetitively with precision. Within the past week we were able to replicate the results Kilean achieved back in March with capturing gold particles in the membrane using tangential two-pump flow. 80…
I know this is my third post today, but this is not a data post. It’s more of a PSA. If you want to add beautiful equations into your post (like I did here) then rather than generating a bunch of images and inserting them into the post, you can simply use . is the…
This data is from about a month ago and it something that Jim has been asking me to put up, so here it finally is. When Tejas runs his experiments, he usually leaves me the last mL of blood that he has from his draw in case I want to do anything with it. One…
Last summer, Tejas and I spent a few days beating our heads against the wall because I was trying to develop a mathematical model of particle capture. Specifically, I was trying to understand what amount of transmembrane pressure we would need to hold a particle on (or in, as we are a politically correct, non-discrimination…