I am trying to grow endothelial cells under flow and measure electrical resistance of the culture during the flow-based growth. I am using a peristaltic pump to generate the flow. I am using microporous membrane with 3 micron pores with 3 micron spacing, and 20% porosity for this experiment. Microporous membranes can facilitate neutrophil transmigration,…
My last experiment did not go as planned, but the results are very interesting nevertheless, and might provide another interesting application for these tw0-membrane systems that we had not yet considered. The start of the experiment went as planned: painted PDMS on the chip, used additional PDMS to glue the gasket to the PDMS layer,…
I made a size ladder of ALD-ed chips. The chips I began with were ~35 nm average pore size, and I applied 2, 5, 7, 10, and 12 nm of alumina using the ALD in UR nano. This does represent an incomplete data set – the 7 nm and 12 nm alumina chips I first…
Program C. Gu – Harvard Seminal Experiments Reese and Karnolvsky 1967 Brightman and Reese (1969) Two unique features of CNS endothelium: Specialized tight junctions – Very low rate transcytosis. Tight Junction by EM Ben-Zvi et al 2014 Nature. Transcytosis becomes upregulated not tight junctions. 2-3x injection HRP injection. Tracer containing vessels travel in endothelial cells…
Josh Miller and Elizabeth Hirschman (RIT co-op student working on STTR lift-off project) obtained preliminary successful results lifting off ~1 in2 sections of NPN membrane where the nanopores were only transferred into the nitride layer in a microporous pattern (ø3 µm pores, hex-close-packed on 6 µm pitch). Details of process are given below. Bruce…
Xingyu et al; Supplement The authors of this submission to ACS Nano apply techniques for the creation of mesoporous silica thin films with vertically aligned nanochannels to create ultrathin (< 100 nm) silica membranes with ~ 2 nm nanochannels. The technique involves the growth of silica around surfactant templates followed by the removal of the…
Based on the talk I gave at the ICNMM (International Conference on Nano, Micro and Minichannels) this past summer, I’ve been asked if I would organize and chair a session titled: Transport in Membranes and Nanofluids. I’d like to include people from NRG as well as membrane collaborators. Consider keeping these dates open: July 10-14, 2016. The conference…
Notes from the ECM4 conference Met with Mathew Halls from Schrodinger. He was curious about what I did, came up, gave me his card. Apparently he knows Chin Tang. He’s giving a talk on the last day. Opening Remarks – Bob Collier – Aberdeen Chamber of Commerce European Capital of Oil and Gas. 25% of…
I decided that instead of trying to figure out why it was delaminating, I would simply remove the option altogether.To that end, I painted PDMS on two NPN chips up to the oxide spacer. Attempt 1: This chip was one I laminated myself using my breath to contact the membranes. In case it’s unclear,…
I am doing a few more membrane transfers and trying to troubleshoot my delamination problems, and I wanted to run my protocol by you guys before I mess up any more chips. I was able to get about 3/7 transfers visibly very good without any wrinkles, but they all still delaminate, either during the fabrication…