Name Place Email Description of Interests Status of Discussion Pierre Olivier, organizer Paris pierre-olivier.couraud@inserm.fr No contact Nicola Marchi (Luca’s friend) Montpellier, France Nicola.Marchi@igf.cnrs.fr No contact Lester Drewes (Organizing committee) U Minn (organizing committee ldrewes@d.umn.edu exosome islation from plasma No contact Yuri Persidsky Temple Univ., PA yuri.persidsky@tuhs.temple.edu No contact Jane Alder UK jealder@uclan.ac.uk No contact Imola…
A quick update since we last talked about how DNA controls were proving to be problematic: Things are working again (more or less). I went back top basics, remade most of my solution, ordered new DNA samples, and, most importantly, piranha-cleaned my chips. The first three experiments I did on basic 30-nm thick 5.4mm square…
Purpose: To investigate the formation of focal adhesions (FAs) on various cell culture substrates, including TCP, SiO2 membranes, and PDMS membranes. Background: FAs are large macromolecular assemblies through which mechanical force and regulatory signals are transmitted between the extracellular matrix (ECM) and an interacting cell. FAs consist of a complex network of ECM, membranes, and cytoplasmic components…
After all the nafion-coated pnc-Si chips that Jirachai gave me broke during my last attempt to filter salt water, Jirachai was kind enough to supply me with a new batch of chips, this one a mix of SiN and pnc-Si, both covered in a thin film of nafion. The idea was that since Nafion is…
I followed Greg’s example and embedded a broken-membrane chip in a PETL. The idea was to use it as a negative control before I use an intact chip. Laminar flow should be maintained in this scenario in order for us to conclude that the membrane is responsible for mixing, should it occur. 4 layers…
To analyze the dimpled Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples. We’ve already shown that cells will grow on these membranes in our “Dimpled Sylgard Membrane Device” post and that our substrates are wetting in our “Degassing Experiments”…
As we were conducting our experiments on dimpled Sylgard substrates we knew that another micro-topography that we wanted to investigate was micro-pillars. In like manner to the dimples, a post microtopography should provide some sort of surface for the cells to “grab onto” and spread as they would on any ECM in vivo. These…
Hi all, A while back we have shown that geltrex coating enhances the attachment of HUVEC on SiN membrane . Jim believes that geltrex may be extended an “all-purpose” coating to promote the adhesion of different cell types to the SiN membrane, but worries that the geltrex coating maybe too thick (e.g. tens of microns), which…
We’ve had quite a bit of success transferring nanoporous membranes now, but a lot of questions about the exact nature of the physics at work during the transfer process remain. Pursuant to those questions, we wanted to try changing a variable — switching from nanopores to micropores — to see how that changes the behavior of…
Adding pockets (250 micron long, 76 micron deep) to the bottom of the channel (channel is 500 microns wide, 125 deep) in a 4 layer PETL (1 inch long chip) promotes disruption of laminar flow.