Recently, SiMPore has been working on a new chip design which involves incomplete etches on both sides in order to create a fluidic system built directly into the silicon, consisting of many long, shallow trenches. These trench chips are designed to obviate the need for silicone gaskets on one side of the chip, reducing the…
9:10 – 9:55am Tony Pietropaoli Must drink liters of water but not urinating. Retaining. Massive edema. Cases that recover must absorb 1/3 to 1/2 of all patient deaths in hospital are related to sepsis. May be an underlying cause but sepsis triggers the hospitalization and causes the death. – Liu JAMA 2014…
pdf of Zydney Paper This paper tackles the concept of membrane capacity – the amount of fluid a membrane can pass before it’s flux declines (due to pore blockage or the buildup of a cake) to some arbitrary fraction of the initial flux. Common practice in industry (according to the paper) is to calculate capacity…
Hi all, A while back I have posted about my attempt to culture bEnd3 (mouse brain endothelial cell) underflow, which failed to maintain bEnd3 proliferation and did not promote alignment under flow. As an alternative/control, I switch the cell types to HUVEC (human umbilical cord endothelial cells), which is known to do well under flow….
Our collaborator Fernando has made a really nifty way to make microfluidics work with lamination pouches. These flat pouches can be sealed together using a heat activated glue, which can be done consistently using a 15 for a 100-pack of 8.5″x12″ pouches + $40 laminator Put multiple materials and multiple chips in the same microfluidic package….
Continuing my work from last week, Tucker used the same tenting (NPN, vapor transfer) and incubation procedure (20 nm and 60 nm Au particles) on an oxide substrate with posts (200 nm high). The substrate was dessicated in the 70C oven overnight. No additional platinum or gold coatings were made. The sample was tilted slightly…
As seen in prior NRG post, ozone treated samples recover over about 24 hours. However, recent experiments at Gaborski Lab show high variability when using the Corona wand to treat samples with ozone. The goal of this experiment was to determine the contact angles of PDMS samples when exposed to ozone treatment. PDMS samples were…
Summary: I have some ok images (20x) of cells growing on MgF2. My controls still look poor. Something about the stain is causing the TCP + NPN controls to detach ahead of the MgF2 material, even if they aren’t fully dead yet. I used acetone fixation, which worked well for the materials on the nanomembrane, but…
I wish I had read Tucker’s lat post before trying this, but since I just got the steam jig from the machine shop I wanted to try it out. My mistake in the two transfers I attempted was that I applied the steam evenly as opposed to the suggested 45 degree angle, but the results…
To test the filtration capabilities of our tents, Tucker and I immersed a tented (wafer 1150, ~30 nm pores) oxide structure (~3 um hole diameter, 200 nm high) and a nontented structure in a solution containing 60 nm (.043 nM) and 10 nm (~10 uM) Au nanoparticles for 1 hour (2 uL incubation volume), rinsed them…