Kevin was kind enough to share his presentation with us from his visit this July:   Here is the link on Owncloud:   http://drive.bbe.23a.myftpupload.com/public.php?service=files&t=c3eceae62439a478b4d1b676924e45ee

We’ve been doing a lot of work characterizing SEPCON performance in forward and reverse configuration.  One lesson we’ve learned is that a vertical orientation might be best for high particle concentrations.  I discussed this with Greg and he graciously came up with first prototype design.  Greg will put is 3D printing experience to work on…

I posted previously about an issue we have with needing to strip the native oxide from the sacrificial silicon layer prior to performing the through-pore etch with XeF2 that enables separation of the oxide membrane from the wafer substrate.  The issue is that the membrane is generally attacked by whatever etch method is used to…

This is a followup to my previous post, found here. Some of the data in that post is now outdated and will be replaced with newer data here. I’ve added to the comparison the diffusion of cytochrome C and urea through a polyethersulfone “high-flux” membrane. This material is commercially available just like the cellulose triacetate….

We borrowed a method first published by Jahn et alii about 7 years ago, in which lipid dissolved in ethanol are streamed through a central channel and compressed by 2 streams of  buffer (we used PBS).  This hydrodynamic focusing results in the generation of liposomes at the interface of the miscible liquids.  This results in…

In this post I address every objective we started with and summarize our results: a) Building of research capacity at Fisher. —–> We would like to to produce our lipid-based nano particles in a cheaper and more reproducible way. For the last 4 weeks Ryan has been producing phosphatidylcholine liposomes.  Depending on the rate of flow, we…

Overview: After trying an initial experiment of growing ADSCs on TCP and 3um translucent (TE) membranes and then placing them onto TCP or 3lp SiO2 membranes, it was determined that further studies should be conducted. For this experiment we grew ADSC on TCP, 3um translucent (TE) membranes, and 0.4um transparent (TE) membranes. Once the cells…

Overview: Bob etched 4 non-porous membranes, on the backside of the membrane. HUVECs were to be grown on these etched non-porous SiO2 membranes and compared to unetched non-porous membranes. Below Bob describes how the membranes were etched: “Four of the non-porous SiO2 membranes were placed on a ø6” carrier wafer with the membrane facing up in the…

Overview: The study of ADSC spreading on various surfaces was conducted to determine if porous surfaces and the porosity of the surfaces had any effect on cell spreading. Eight different surfaces were studied and each of the membranes were SiO2, these included: 0.5um low porosity (LP), 0.5um high porosity (HP), 3um LP, 3um HP, 5um…

As part of the ongoing study of NPN membrane filtration characteristics I used the SEM to look at membranes after they were used.  Unless otherwise indicated, files with a “t” (top) indicate the chip was membrane side up.  “b” stands for the bottom, or trench side.  In a standard forward centrifuge setup we expect the…