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Nanomembrane Research Group
  • NRG

    BMES 2014 Talk Comments – Greg

    ByGreg Madejski October 27, 2014

    Here are my thoughts on the talks I attended at BMES: OP-Thurs-1-11. BioMEMS I Oral Thursday, October 23, 2014 8:00 AM – 9:30 AM Room 008A Moderator 1: James Tunnell Moderator 2: Catherine Klapperich 8:15 AM – 8:45 AM High-throughput High-content Developmental Biology and Neurogenetics H. Lu. Abstract This talk used a number of image processing…

    Read More BMES 2014 Talk Comments – GregContinue

  • NRG

    First workable clams

    ByGreg Madejski October 7, 2014

    After a lot of trial and error with the hinge, I was able to create something usable!!! Apparently I cannot upload the .stl file or .skp file to the blog directly for “Security Reasons”   I printed this one out of Makerbot ABS at 228 C (build plate 115 C), with 0.15 mm layer height slicing,…

    Read More First workable clamsContinue

  • NRG

    Optimization of HUVEC Network Formation in Geltrex On and Off Membranes

    ByRandi del Rosario October 6, 2014October 6, 2014

    Our motivation was to look into how ADSC influence HUVEC network formation. My first objective was to investigate the optimal cell count, the best volume of Geltrex and if it was better to use M200 media with or without growth supplements. I then looked into seeding HUVEC on a nonporous membrane. At the same time,…

    Read More Optimization of HUVEC Network Formation in Geltrex On and Off MembranesContinue

  • NRG

    Makerbot Replicator 2x Open for ABS prints

    ByGreg Madejski October 6, 2014September 24, 2020

    Recently, Jim has purchased a Makerbot 2x printer for our group. I’ve spent the last few weeks printing and debugging. It has the capability to print many different types of material, along with 2 separate nozzles. I have been able to get many fine prints done with ABS plastic, and I’ve put together a basic…

    Read More Makerbot Replicator 2x Open for ABS printsContinue

  • NRG

    First Flow Chip Pictures

    ByGreg Madejski October 3, 2014

    We’ve been moving to a new format for chips, trying to standardize an active area that has good yield and allows fluidic access through the chip itself. The SEPCON outer dimensions have been reused (5.4 mm x 5.4 mm). It’s a 1.5 mm^2 single slot chip area (2 mm x 0.7 mm) with vias that…

    Read More First Flow Chip PicturesContinue

  • NRG

    Variation of trans endothelial electrical resistance (TEER) with different membrane configurations

    ByTejas Khire September 28, 2014

    A bit of background: In order to quantify how permeable a tissue barrier is, we use a method known as trans epithelial electrical resistance (TEER). Cells are grown on a permeable growth substrate in a transwell format. When the cells are not confluent, there is a continuous diffusion of ions across the two sides of…

    Read More Variation of trans endothelial electrical resistance (TEER) with different membrane configurationsContinue

  • NRG

    Zeta Potential measurements with MgF2 nanomembranes

    ByGreg Madejski September 22, 2014

    I believe that we can use MgF2 nanomembranes as electroosmotic pumps, along the same lines as pnc-Si and NPN materials. To first get a sense of how much pumping I would expect, I wanted to know what the zeta potential of these membranes would be, as the potential is directly proportional to the amount of…

    Read More Zeta Potential measurements with MgF2 nanomembranesContinue

  • NRG

    More separations using wafer # 1142 with 35 nm pores and 14% porosity

    ByJosh Winans September 21, 2014

    In our last post we showed that this latest wafer with 36 nm pores did a better job of blocking 20 nm NPs in the forward and reverse orientations, whereas an older wafer (1070- 45 nm, 15%) showed better separations in reverse orientation.  Here is a couple plots summarizing the difference: So 20 nm NPs…

    Read More More separations using wafer # 1142 with 35 nm pores and 14% porosityContinue

  • NRG

    Report on Gordon Fuel Cell Conference

    ByBob Carter September 15, 2014

    I attended the 2014 Gordon Research Conference (GRC) on Fuel Cells in August. For those of you not familiar with Gordon conferences, I will make a plug for them as a great venue for learning much detail and interacting closely with many of the major contributors in a specific field. GRC has a wide variety…

    Read More Report on Gordon Fuel Cell ConferenceContinue

  • NRG

    Ultra-Thin Self-Assembled Protein-Polymer Membranes: A New Pore Forming Strategy

    ByThomas Gaborski September 11, 2014September 11, 2014

    I recommend we review this article in NRG or journal club – it was just published online in Advanced Functional Materials this week. I see a lot things in this paper that appear to be motivated by our work. They showed SFM/AFM membrane thickness step height, NP gold separations (and cake formation) and water permeation/permeability. Unfortunately for…

    Read More Ultra-Thin Self-Assembled Protein-Polymer Membranes: A New Pore Forming StrategyContinue

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    • Home
    • Publications
    • Membranes
      • Common Chip Formats
      • Common Membranes
      • Microslit Membranes
    • Devices
      • µSiM
        • Geometry
        • µSiM CAD Files
        • Assembly
          • Protocols.io (µSiM Assembly)
          • Instructions
          • Common Issues and Troubleshooting Tips
        • Cell Culture Protocols
          • Top Well: hCMEC/D3
          • Top Well: HUVEC
          • Bottom Channel Culturing
          • Immunocytochemistry Protocol
          • Impact of Chip Orientation on Fluorescence Imaging
          • Permeability: In Situ Method
          • Permeability: Sampling Method
          • Cell Culture Common Issues and Troubleshooting Tips
      • SepCon®
        • Sepcon Assembly
        • Sepcon Video Protocol: Assembly
        • SepCon Gasket Silhouette File
        • SepCon Video Protocol: Wetting the membrane
        • SepCon Video Protocol: Disassembly
      • µSiM-DX
        • µSIM Video Protocol: Capture of Nanoparticles
    • Impact
      • TraCe-bMPS
      • HCIC
      • LOMP
      • SiMPore