SepCon® Video Protocol: Wetting the membrane
Below is a video protocol for wetting a sepcon device. There is no audio but amounts are detailed.
Below is a video protocol for wetting a sepcon device. There is no audio but amounts are detailed.
Ring Resonators are photonic structures that have a very repeatable behavior. As light is guided along a path, it can evanescently couple into a very closely positioned wire (submicron gap). If the wavelength of light is just right, constructive interference can occur, causing the rings to resonate (lost through scattering). Necessarily, this causes a drop…
Hi all, This post showcase a simple (low-tech) alignment tool for assembling the different components of a microfluidic chamber. Essentially, the alignment pieces are made from two index papers cut using the Silhouette Cameo. The two cut index papers are bonded together using double-sided tapes. The square holes are where the membrane chips are to…
Vincent has asked us to integrate our nanoporous filter on top of a thin layer of silicon nitride, so that he can clean up his analyte before being exposed to his single nanopore (fabricated using a dielectric breakdown). There are many different ways to join silicon wafers together. Specifically, we wish to create the following…
Lately I’ve been working on building computational models which can be used to make predictions about the behavior of a diffusion experiment, to allow for the collection of large pools of data without the difficulty and time consumption associated with actually performing an experiment. This could prove to be a valuable tool for the continued…
I’ve been trying for some time to make chips that can dynamically change their surface charge. To this end I’ve made the following NFT chips, which have 12 nm of silver coated in 10 nm alumina: Note the Aluminum foil coating the insides of the gel box. The silver layer is either photoactive or…
In a previous post (link) I presented a video that showed that the voltage applied to a silver film changed the pumping behavior of a membrane. The key data is summarized by the following powerpoint slide: My most recent post (link – electrical characteristics…) showed, however, that the breakdown voltage of these silver and alumina NFTs is…
In recent posts we have tested the total volume of filtrate as a function of time in the centrifuge. We used NPN with different average pore sizes and we studied 100 nm and 20 nm NPs as well as IgG. We showed that while 100 nm NPs were completely blocked 20 nm NPs and the…
Just before BMES I was finally able to electrically characterize my NFT chips. Previously, I had been trying to collected IV sweeps using the following circuit: The problem with this was that, as Jess discovered, the membranes have almost no electrical resistance in this configuration, and I was essentially shorting out the circuit. This meant…
In response to last meeting: We tested more controls to help support our idea that the ADSC are migrating toward HUVEC network and prematurely breaking them down. We used a 100% Geltrex coated cytovu gasket to test if ADSC are attracted to something in the Geltrex causing them to migrate. The ADSC did not migrate…