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Nanomembrane Research Group
  • Protocols (Public)

    SepCon® Video Protocol: Wetting the membrane

    ByKarl Smith November 3, 2014December 28, 2022

    Below is a video protocol for wetting a sepcon device. There is no audio but amounts are detailed.

    Read More SepCon® Video Protocol: Wetting the membraneContinue

  • NRG

    Silicon Photonics Integrated onto SimPore Chips

    ByGreg Madejski November 3, 2014

    Ring Resonators are photonic structures that have a very repeatable behavior. As light is guided along a path, it can evanescently couple into a very closely positioned wire (submicron gap). If the wavelength of light is just right, constructive interference can occur, causing the rings to resonate (lost through scattering). Necessarily, this causes a drop…

    Read More Silicon Photonics Integrated onto SimPore ChipsContinue

  • Knowledge | NRG

    simple alignment tool for microfluidic assembly

    ByHenry November 2, 2014November 2, 2014

    Hi all, This post showcase a simple (low-tech) alignment tool for assembling the different components of a microfluidic chamber. Essentially, the alignment pieces are made from two index papers cut using the Silhouette Cameo. The two cut index papers are bonded together using double-sided tapes. The square holes are where the membrane chips are to…

    Read More simple alignment tool for microfluidic assemblyContinue

  • NRG

    Anodic Bonding for Flip-chip Microfluidics

    ByGreg Madejski October 31, 2014October 31, 2014

    Vincent has asked us to integrate our nanoporous filter on top of a thin layer of silicon nitride, so that he can clean up his analyte before being exposed to his single nanopore (fabricated using a dielectric breakdown). There are many different ways to join silicon wafers together. Specifically, we wish to create the following…

    Read More Anodic Bonding for Flip-chip MicrofluidicsContinue

  • NRG

    Computational modeling of diffusive transport

    ByTucker Burgin October 31, 2014November 5, 2014

    Lately I’ve been working on building computational models which can be used to make predictions about the behavior of a diffusion experiment, to allow for the collection of large pools of data without the difficulty and time consumption associated with actually performing an experiment. This could prove to be a valuable tool for the continued…

    Read More Computational modeling of diffusive transportContinue

  • NRG

    A New Platform for Pressure-Driven Voltage-Gated Separations

    ByKarl Smith October 28, 2014January 22, 2015

    I’ve been trying for some time to make chips that can dynamically change their surface charge. To this end I’ve made the following NFT chips, which have 12 nm of silver coated in 10 nm alumina:   Note the Aluminum foil coating the insides of the gel box. The silver layer is either photoactive or…

    Read More A New Platform for Pressure-Driven Voltage-Gated SeparationsContinue

  • NRG

    Surface-Charge-Dependent EO Pumping Behavior Persists (weakly) at Low Applied Voltages

    ByKarl Smith October 28, 2014January 22, 2015

    In a previous post (link) I presented a video that showed that the voltage applied to a silver film changed the pumping behavior of a membrane. The key data is summarized by the following powerpoint slide: My most recent post (link – electrical characteristics…) showed, however, that the breakdown voltage of these silver and alumina NFTs is…

    Read More Surface-Charge-Dependent EO Pumping Behavior Persists (weakly) at Low Applied VoltagesContinue

  • NRG

    Modeling pore clogging

    ByJosh Winans October 28, 2014

    In recent posts we have tested the total volume of filtrate as a function of time in the centrifuge. We used NPN with different average pore sizes and we studied 100 nm and 20 nm NPs as well as IgG. We showed that while 100 nm NPs were completely blocked 20 nm NPs and the…

    Read More Modeling pore cloggingContinue

  • NRG

    Electrical Characteristics of Silver/Alumina NFTs

    ByKarl Smith October 28, 2014January 22, 2015

    Just before BMES I was finally able to electrically characterize my NFT chips. Previously, I had been trying to collected IV sweeps using the following circuit: The problem with this was that, as Jess discovered, the membranes have almost no electrical resistance in this configuration, and I was essentially shorting out the circuit. This meant…

    Read More Electrical Characteristics of Silver/Alumina NFTsContinue

  • NRG

    HUVEC-ADSC Co-Culturing on Membranes

    ByRandi del Rosario October 27, 2014

    In response to last meeting: We tested more controls to help support our idea that the ADSC are migrating toward HUVEC network and prematurely breaking them down. We used a 100% Geltrex coated cytovu gasket to test if ADSC are attracted to something in the Geltrex causing them to migrate. The ADSC did not migrate…

    Read More HUVEC-ADSC Co-Culturing on MembranesContinue

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    • Home
    • Publications
    • Membranes
      • Common Chip Formats
      • Common Membranes
      • Microslit Membranes
    • Devices
      • µSiM
        • Geometry
        • µSiM CAD Files
        • Assembly
          • Protocols.io (µSiM Assembly)
          • Instructions
          • Common Issues and Troubleshooting Tips
        • Cell Culture Protocols
          • Top Well: hCMEC/D3
          • Top Well: HUVEC
          • Bottom Channel Culturing
          • Immunocytochemistry Protocol
          • Impact of Chip Orientation on Fluorescence Imaging
          • Permeability: In Situ Method
          • Permeability: Sampling Method
          • Cell Culture Common Issues and Troubleshooting Tips
      • SepCon®
        • Sepcon Assembly
        • Sepcon Video Protocol: Assembly
        • SepCon Gasket Silhouette File
        • SepCon Video Protocol: Wetting the membrane
        • SepCon Video Protocol: Disassembly
      • µSiM-DX
        • µSIM Video Protocol: Capture of Nanoparticles
    • Impact
      • TraCe-bMPS
      • HCIC
      • LOMP
      • SiMPore