The Long Road to Wearable Dialysis

As a step to identifying the particles that I am capturing in my systems, I am attempting to do an immunogold labeling with a CD 63 primary antibody and a 10 nm gold conjugated secondary. It is possible to run this analysis in an SEM, but the best confirmation method is using a TEM and…

Summary: The fabrication of NPN relies on the formation of pnc-Si over a non-porous SiN film, then etching the pattern of the pnc-Si into the SiN. I used the same concept to replicate the dual-scale nanomembrane style (microporous+nanoporous) featured in Alec’s work, as well as create a porous/nonporous nanomembrane substrate, by using existing SiN or…

I drew an approximation of the SiMPore liftoff device and ran the COMSOL model with 1 mL/min urea fluid flow in the upper channels and 2 mL/min dialysate in the bottom channels. With this initial model, the urea was cleared to 40% (60% removed). This is an instantaneous urea clearance rate of K= 0.6 mL/min….

I dug out some of my old data and found this comparison for 3x and 6x window flow rates at different voltages (minimal repeats, error bars not included): This shows that as voltage goes up, the flow rate increases for each of the active areas shown here.  Obviously if we were to publish this we’d…

One of the other suggestions from Angela Glading at lab meeting a while ago was to quantify the vacuoles on different culture areas.  So, I selected images from 3 different experiments (all at day14 of growth) and counted the number of cells which expressed vacuoles on different areas.  I then used my mask code in…

Tejas found this…  A cool article and video on water ‘sticking’ the surface. Notice the difference in advancing and receding angles. http://web.mit.edu/newsoffice/2013/droplet-surface-adhesion-0219.html