The Long Road to Wearable Dialysis

One of the major goals of my work with capturing exosomes is to show that our purified population is less contaminated with protein than exosomes purified by ultracentrifugation. This is important because the protein (and all the other junk floating in plasma such as cfDNA) can cause problems with identification and characterization of the exosomes….

Carrying our membrane functionalization work forward we attempted to work in both the polymer chemistry space and the KODE nonspecific binding space. With the results shown below the KODE molecule pathway has shown much more promise than the polymer chemistry pathway. This, for the moment, has provided the conclusions that fuel the title of this…

The following is a plot of rhodamine diffusion for the experimental system at various time point: In this figure, the rhodamine approaches equilibrium, as defined as a filtrate/retentate value of 1.  The 9x well chips have a larger active area and quicker diffusion. I have performed simulations of this system using COMSOL, and in this…

In this post I take a look at the flux of molecules through the 1-D membrane simulations.  I will then use this flux to compare to systems lacking a membrane. First off, how to find the flux.  Inside the membrane, the Flux = -Dm dc/dx.  Unfortunately this is extremely hard to calculate because of the…

This was something I set up a few days ago and finally got a chance to run the gel.  I was curious to try this after the previous forced separations using brain extract.  If you recall, the “separation” using the pressure cell did not have a visible cutoff and all of the bands appeared in…