Lab Specific Training

Hello Everyone,   So first of all, a quick reminder of what i have been working on: we were able to show the trapping of the beads by TE membranes, although in final step and after releasing of the beads, we could still see beads on the membrane, so we hypothesized that the beads are…

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as the seeding density.  I always had 3 samples – bEnd3 alone, NG10815 alone and the…

Intro For the past couple months, I have been working with Tejas to recreate his previous experiments using a more complex system. The goal of the research is to study the transmigration of neutrophils and represent how they behave during chronic inflammation in vitro. To do so, we planned to use a setup that included…

Hi all, After experimenting with UV ozone bonding of PDMS to glass, Si, and pnc-Si for the past few weeks with Mike, we have reached the following conclusions: 1. The bonding of PDMS to glass is always stronger than the bonding of PDMS to Si and pnc-Si, given that all receive same treatment of UV…

Previous Posts Anodic Bonding Theory, Anodic Bonding at RIT I’ve put together an anodic bonder setup here at the U of R and will be moving it inside the cleanroom shortly.     I tried a couple of known combinations to see if the bonder was working properly. The general principle is to liberate sodium ions…