Lab Specific Training

Manufacturer’s Data Logged by: Ahmet Gurcan Membrane Type: MSSN 0.5 micron Microslit SKU: MSSN400.C-3LX.1-0.5E1 Lot Number: 250409-13 Quantity Received: 250 Manufacturer Test Report: Flow Data Avg in SLPM 4psi: 3.96 15psi: 14.4 Burst: 9.82 PSI

Here are the results of diffusion based protein separations at different salt concentrations and 50kD Microcons: We see a sharp cutoff between 31 and 45kD.  Separations look like they have progressed further in 10mM salts rather than 100 mM (this is the opposite of what we see in pnc-Si).  The 100kD data may also support…

I ran some tests today using the 0.01um, 0.05um, and 0.10um pore diameter track-etched membranes from sterilitech. The tool the paul built to cut the membranes works very well, and assembly into the microcon systems it simple. I used the standard vertical-wall o-rings that come in the microcon systems because based on yesterdays test results…

I did an experiment earlier this week in the Sepcon and flat format. I intentionally broke membrane windows so that I could compare chips with no intact membranes vs. chips with 1 intact membrane (and also the effect of autoclaving). Unfortunately, all of the flat chips discolored overnight, so I couldn’t see any differences between…

To try to calibrate the STED for imaging on our membranes, Bill and I have been trying to use 50 nm silica beads that have been captured on the membrane. However, we discovered initially that the beads were actually not too easy to work with and clumped like crazy. On STED, we weren’t really able…

Objective Develop an easily mainpulated reproducible vasculogenesis assay using hydrogels laden with endothelial cells (‘bio-ink’) patterned using the technique of extrusion bioprinting Bio-inks Develop a bio-ink with the following characteristics Cell viable: bio-ink must sustain cell life, allowing cells to manipulate and move through the hydrogel Vasculogenic: bio-ink must encourage (or at least not negatively affect) tubule formation…