WHERE: URMC ** Room # 4-7545 **
WHEN: Tuesday April 16th & Wednesday the 17th.
TIME: 9:00-4:00 P.M.
Please drop off your pipettors early as we expect a good turnout.
Pipettors will be done on a first come, first served basis. Thank you!
I made a lot of 6 devices for Vaccinex. We attempted to seed human endothelial cells on the membranes, flowing at 20 uL/min, first coating the surface of the membrane with an adhesion molecule at 20 uL/minute. At the same time, the same cell line was grown in a 24 well TEER z-scope tool to…
I am trying to grow endothelial cells under flow and measure electrical resistance of the culture during the flow-based growth. I am using a peristaltic pump to generate the flow. I am using microporous membrane with 3 micron pores with 3 micron spacing, and 20% porosity for this experiment. Microporous membranes can facilitate neutrophil transmigration,…
Last week, I deposited silicon nitride/ amorphous silicon/ silicon nitride stack and then annealed the free standing stack after etching the substrate. The nitride film was deposited with 25W bias and the silicon film with 5W bias. The thickness for the nitride film is 30nm where it is 25nm for silicon. One of the initial…
I’ve made some modifications to my V2 design such that it allows total vertical access, among other things: This design utilizes embedded electrodes and filter within gasket layers to create a tight fluidic seal for each channel. Here, the slot is pointed upward, so the endothelials will be on the bottom side and the…
Introduction Here, I will post figure drafts for the paper that introduces the modular µSiM (m-µSiM). Figures Remaining data to obtain: Occludin staining in IMR90-4 from UniBe in µSiM. We have tried this after 2 day culture in µSiM, which worked at UR but is not working at UniBe. Current problem: Occludin is not seen in…
Summary: Dan and I went to Woods Hole Oceanographic Institute to learn about the current state of microplastics research. There were a number of things that we learned that we did not know. The common sentiment among researchers agreed climate change was a much bigger threat to humans than microplastics, but the reality and immediacy…