Large-area Ultra-thin Membranes Using XeF2 Senior Design
Power Point for my Senior Design
https://docs.google.com/file/d/0B1AH05-mf_tQLTdSMHRWX0NjNGc/edit?usp=sharing
Power Point for my Senior Design
https://docs.google.com/file/d/0B1AH05-mf_tQLTdSMHRWX0NjNGc/edit?usp=sharing
After experimenting with some different coatings and membrane types in single-well CytoVu devices, here are some representative images of the results: SiN (Nitride) membrane with a Geltrex non-gel coating @ 44hr: 10x: 20x: 40x: SiO2 (Oxide) membrane with a Geltrex non-gel coating @ 42hr: 10x: 20x: 40x: Also, some Live/Dead Assay images were taken on…
Dan Mendelson performed gold separations using wafer 255 and has shown that this wafer has a cutoff for gold near 25 nm. Below I have plotted the data of filtrate gold concentration as a function of the feed concentration for the three wafers we have tested. The bottom panel is a replot of the Malvern…
Given the results of my previous post, I am reworking my design to make it more manufacturable. BBB Device V2-6 Compressed The major change compared to my last design is the inclusion of a full length microscope slide and coverslip. Instead of cleaving the electrodes from the glass, this step eliminates the need to…
Now that we have preliminary transport data from the Tecan, we need standard curves in order to get permeability coefficients. This is really tricky because the RFU (relative fluorescent units) are arbitrary and based on the Tecan gain. For example, a solution of 10uM rhodamine might be 5000 RFU with a gain of 80 but…
Introduction In the Glading Lab we study Cerebral Cavernous Malformations (CCMs). In CCMs, brain capillaries develop enlarged lumens and a disrupted blood-brain barrier. The presence of a mutation in KRIT1 causes loss of KRIT1 protein function in the endothelial cells. KRIT 1 plays a role in the integrity of endothelial junctions, therefore the lack of…
Originally, I created the following figure to visualize the relationship between sieving coefficient and molecular weight: (originally from this post) I can now take the protein dimensions that I obtained from crystal structures (posted here) and replot this figure: In this version of the figure the sigmoidal shape of the curve is even more obvious…