This paper details a PDMS microfluidic system to perform dose response assays (what I’m hoping to achieve over this summer). They use serial dilutions to get separate trials spanning six orders of magnitude, showing an optimal dosage to kill HeLa cells. Their system is very efficient, using very shallow channels and balancing the fluid flux across all channels with a highly resistive design.
Continuing my work from last week, Tucker used the same tenting (NPN, vapor transfer) and incubation procedure (20 nm and 60 nm Au particles) on an oxide substrate with posts (200 nm high). The substrate was dessicated in the 70C oven overnight. No additional platinum or gold coatings were made. The sample was tilted slightly…
I spent some more time in understanding the numbers I am getting for our device simulations. In my simulations, I am assigning a conductivity value for my cell layer. Technically, from this information and by knowing the thickness and area of the cell layer, I can analytically calculate the value of resistance that the cell…
A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given that there is a parallel project with membrane masking of ECM with parylene stencils, we…
Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells. Summary/Conclusions: In this follow up post, I seeded adipose derived stem cells on 50 nm thick microporous SiN liftoff membranes with…
With some of the recent high porosity membranes being produced, I wanted to demonstrate that we can use ALD to create a movable cut-off without sacrificing too much in permeability. I took sc713 and coated it with 5 nm and 10 nm of Al2O3 i.e. I shrank the pore diameter by 5 and 10 nm….
Ring Resonators are photonic structures that have a very repeatable behavior. As light is guided along a path, it can evanescently couple into a very closely positioned wire (submicron gap). If the wavelength of light is just right, constructive interference can occur, causing the rings to resonate (lost through scattering). Necessarily, this causes a drop…