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Using Alumina Deposition to Modulate Pore Size
The Cambridge Microsystems Savannah 200 atomic layer deposition (ALD) system uses a two-step chemical process that alternates pulses of water vapor and trimethylaluminum (TMA) to build a layer of Al2O3 on a silicon substate. Because of the two-step nature of the process, the film is forced to grow by successive monolayers, each with a thickness…
Microporous Membrane Transfer
We’ve had quite a bit of success transferring nanoporous membranes now, but a lot of questions about the exact nature of the physics at work during the transfer process remain. Pursuant to those questions, we wanted to try changing a variable — switching from nanopores to micropores — to see how that changes the behavior of…
Fabricating Thin Polymer Coatings to Study S. Aureus Durotaxis – PUA Optimization
Introduction The work in this post explores the hypothesis that durotaxis affects the invasion of S. aureus into the osteocyte lacuna-canicular network (OLCN). To address this hypothesis, we modified the µSiM to enable the independent tuning of pore stiffness, ranging from those of soft-tissues to that of silicon nitride (~10X stiffer than bone). More background…
Impedance Spectroscopy to study neutrophil migration induced permeability
So, I have been working with a 4-probe TEER measurement system to record the changes in endothelial permeability during neutrophil extravasation and other biochemical assays such as thrombin treatment and TNF stimulation. 4-probe systems are sensitive because they don’t suffer from the contact impedances and measure only the potential drop across the sample under consideration. See…
Immune Imaging Symposium Abstract Figure
ByKileanSo Alec, Tejas and I will have a poster at the Immune Imaging Symposium in the Med Center on Nov. 4th and we created an image for our abstract that Jim requested I post here in higher quality. Immune Imaging Abstract Image_01 The image is so high quality that it was having trouble loading into…
Fibroblast wound healing with small EVs
Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which are collected in the last step of the isolation process. I began by seeding human…