Aline Devices for Cell Culture

UPDATE: Some data presented here is out of date. For up-to-date data, see my next post here. — Lately I’ve been performing comparison experiments between our NPN membranes (wafer 1132, 3 slot SEPCON, 40-48nm pores and 13-20% porosity) and commercial cellulose triacetate filtration membranes, with respect to the degree to which they hinder the diffusion…

After doing all the water permeability with this wafer, I saved the samples to do some protein studies. When I write my own paper I will need two wafers to separate protein standards by diffusion and flow to show movable cutoffs. I will also include a brain extract separation to make the point that you…

Hey all,   So, I’ve been trying to replicate the exosome experiments that I did back during my rotation to varied success. I have had trouble with the plasma that we had frozen with precipitates that would not resuspend upon thawing. We ordered new plasma though so fresh batches of experiments will be coming up very…

ALine Capture Device In this post I will go over some of my recent work that is a continuation of Kilean’s research concerning extracellular vesicle (EV) capture and characterization. In the past, this was done using a tangential flow filtration (Figure 1) scheme and clamped devices featuring nanoporous membranes (Figure 2). These devices are straightforward…

Here are some more images from my ongoing cell culture project. My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates I added twice the amount of primary antibody on these devices that I usually do (1:100 dilution, instead of 1:200).     High Porosity MgF2   TCP MgF2 Coverglass  

I have created a successfully sealed flow chamber with this current device (V2.4), with the caveat that I sealed off the top chamber and was unable to test leaking from the top to the bottom. Again, the dialysis chips from Dean’s work were used, as well as the same bulk microscope slide electrodes.   Improvements…