Cell Culture on Gradient Microporous-NPN™

ByAlec

Introduction

As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress.

Methods

HUVECs (P3,4,5) were seed within our mimetic and left to grow to confluent for 24 hours. Shear (4.5 dyn/cm2) was initiated and images were collected following 5 hours.

Figure 1. Preliminary data on cell adherence to μ-NPN material. Cell loss in the first 5 hours is most prominent in the first three regions (1:2, 1:3, 1:4).
Figure 2. Data collection and characterization. Results were binary and collective result was used to base my final membrane group selection (n=3).

 

Figure 3. Heterogeneity in shear results a function of pressure?

 

Figure 4. Cell loss under cell material in side by side pattern experiments.

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