Tendon-on-Chip Platform for Modeling Inflammation and Fibrosis in Tendon Injury
This is my draft presentation for my CMSR presentation on December 4th.
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Production updates
There’s been a lot of production in the last month and we’re still sorting through the data, but here is a synopsis of some key findings. All films were deposited with the AJA tool. Film reproducibility Week-to-week variation in pore morphology has never been noted explicitly due to the rapidly changing conditions we place on…
To Pulldown or Not to Pulldown, That is the Question: Testing the Utility of a Pulldown in µSiM-CA Bacteria Assays
The idea of adding a pulldown modification to our bacteria assay protocol came about after we started to believe that pore discovery was our rate limiting step of the transmission process, discussed here. Initially, it seemed to benefit our assay as it bumped our performance up significantly. Upon closer inspection we realized that we had…
Model curves for TE, graphene, and SiN membranes at various pressures
After correcting a minor error in my code that was giving me some weird limiting behavior, and optimizing the code to give me faster results, I generated the following curves: Note that, as experiment predicts, the ‘sharpness’ of the graph increases with increasing applied pressure. The model isn’t exact, and slightly underestimates the actual sieving…
BBEC Pressurize/Diffusion Separations
I wanted to attempt pressurized and diffusion separations of brain extract using the new movable wafers. I tried pressure cell separations of 1:20 BBEC (clarified brain extract) at 2psi for 40 min. The flow rates were better than the standards I was previously using – this could be due to the little bit of glycerol…
Adipose Stem Cells on Nitride LiftOff Membranes (Part 1)
We have successfully plated adipose derived stem cells (ADSC) on microporous silicon nitride (SiN) membranes. Cells plated on nanoporous SiN appeared fine at time of seeding, but membranes were broken at 2 hours – possibly due to media washing or cell spreading/contracting. Josh Miller made new Microporous and Nanoporous Nitride liftoff membranes. These membranes were…
TEM on SiO2 coated membranes
ByJimAs per Jim’s suggestion, I coated wafers 335 and 341 with 40.6nm SiO2 on top and 42nm SiO2 at backside. The membrane cells have been passed to Anant and Maryna for chemical stability and air flow tests. Today I got some TEM images from the medical center with the help from Karen.We first imaged wafer341…