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GO Hemocompatibility Study: I. Hemolysis
ByHenryIntro Recently we have been collaborating with the Moghaddam lab from the University of Florida, which specializes in the fabrication of graphene oxide (GO) membrane, to study the use GO membrane as a toxin filter or absorbent in hemodialysis and live support devices. We are responsible for the hemocompatibility component of the study and used the following…
I-V characteristics of coated membranes
ByJimI measured the I-V characteristics of our coated membranes on Wed. at RIT. JP observed the measurement. The semicon parameter analyzer in the test lab is “old” but isexactly what we need! Roughly speaking, our coated membraneshave resistance about 2k Ohm, depending on the thicknesses ofmetal films (this is based on pure Ti coated samples,…
Variation of TEER as a function of membrane area (COMSOL simulations)
Background: This is a follow up to the blog that Jim wrote on the basics of TEER measurement. This is the link to my old blog, which also describes the theory and some other preliminary data about TEER measurements. The resistance (R) of a wire is the resistivity (ρ) times its length (L) divided the cross sectional area (A) of…
BSA Size/Zeta-Potential and BSA-DDM Complex Results
ByMike HYesterday I used the Melvern to determine particle size and zeta-potential for BSA in DI-water. For the particle sizing I used a concentration of 2mg/mL but I had to increase the concentration to 10mg/mL in order to get an accurate zeta-potential measurment. As shown above the size measurements provided an average diameter of approximately 5.6nm….
Test of Theory– Can we successfully bind Human IgG to the protein A beads and then remove it with buffer B?
Test of Binding/ Unbinding (Carrie and Barrett): Test to see if we can successfully bind Human IgG to the protein A beads and then remove it with buffer B. After ordering the new human IgG and protein A beads, I have been working on a higher-quality analysis of the binding and dissociating of the IgG…
Negative Data for Nanofluidic Transistors, Dielectic ‘Flowers’, and an Interesting Gold Filtering Behavior
I’ve been trying for a year now to make a nanofluidic transistor – a nanomembrane with a thin layer of metal coated in dielectric that allows for the dynamic modulation of the sieving characteristics of the chip. Beginning with the last 14 chips from wafer #1081, I coated them in ~3 nm of Ti and…
