Similar Posts
ASEMV Poster Draft for Feedback
ByKileanHey all, I have the first draft of my ASEMV poster here for any feedback that you might have. The conference is in Monterey, CA next week and any feedback that you have on this by Tuesday or Wednesday evening of this week on the poster would be awesome. ASEMV_Poster_KL_09132017_01
Exploring Urinary EV Detection Using CAD-LB
Introduction This post provides an update on a series of experiments conducted with Sam. These experiments were designed to assess the versatility of the CAD-LB platform for detecting EVs in urine samples. Urine is a complex biofluid often used in clinical diagnosis, making it a suitable subject for testing the CAD-LB’s biomarker detection capabilities. After…
ECMO Model Design
While experimental results are still forthcoming, I thought I’d make a post about the nature of the model I’ve constructed for ECMO. This model is the one that my experiments are seeking to validate; so to be clear, this model is founded entirely in theory for now. ECMO shares some obvious similarities with hemodialysis — blood…
Problem with copper…
There is strong evidence that the recent pinhole breakout is due to copper contamination in our process. I have attached two image – An SEM showing a cuprous creature eating one of our membranes and an EDX (Energy Dispersive X-ray?) spectra identifying it as a copper-rich silicide. This would also explain Dave’s TEM image of…
W613: Retest of Stability in Cell Culture Media
I recently repeated a chemical stability test of RTPed (5 min in Ar at 800C) samples from W613, which has the 30 nm pnc-Si layer. In this study I used DMEM +10% FBS as the culture media without cells. Previously RTP treatment of samples from this wafer survived about 7 days. In this iteration of…
A3G diffusion tests through pnc-Si
This is a summary of diffusion experiments with A3G that were conducted during the week of 5/10/2010-5/14/2010. The HMM structure of A3G is a large protein multimer held together with RNA. Since the ultimate goal of this project is to develop a screening assay for HIV drugs, a successful experiment is one that will show…
2 Comments
Comments are closed.
Beautiful images of aligned cells on the backside of the device! Did you take any images before flow so we can make the claim these are aligned? You mentioned that some cells didn’t survive? Can you explain where?
I might have an image of these cells before flow but it is through the clamp system.I’ll make sure to get those before and after shots when I run this for the AFM measurements. I’ll add to this post next week. The cells that are in the channel of the aline are undergoing much higher shear stresses and the cells get strip off. I noticed that the entrance side seemed to retain more cells then the exit side but maybe it is just me. The membrane is 100% confluent and healthy and I can run two Alines with same system!