Hello everyone, for my first ever post I would like to share with you a collection of ideas that has turned into a review I’ll be working on. Here you will find some selected publications that report interesting fabrication processes at the nanoscale. I have summarized the processes and added some raw schematics of how…
This week I tried looking into several different ways of detecting the elusive Barcikowski Peptide. We discovered that Coomassie staining does not work, both because of the size of the peptide (it only works on proteins with a MW > 3000Da) and because Coomassie preferentially binds basic residues (and with a sequence of CEEEEEEEEEEEEW, we’re…
We still have plenty of things to do, but after five weeks working at the UR, we have come a long way: – Chip production for instructional use: done – Design and production of SU8 mold: done – Protocols for (@Fisher) manufacture of micro and milli (silhouette) fluidic devices: done – Construction and testing of chips…
The mechanism of organic dye photobleaching is not well understood, but there are a few methods out there for reducing the rate of photobleaching. Some literature suggests that photobleaching is due to excess oxygen in solution and the interaction between the dye and oxygen radicals. Actin research swears by the following “anti-bleaching buffer” to limit…
We ordered quantum dots from Invitrogen. These QDs have a PEG coating that terminates in COOH and should cause the dots to be negatively charged. They are about 12.6 nm in diameter: They exhibit a -40mV zeta potential by zetasizer measurements, however the particles appear to aggregate a great deal (under microscope and visibly turn…
Christine and I have been using the present Corning 24-well plate definition in the Tecan for our experiments so far. Yesterday I placed a pnc-Si chip in the (approximate) middle on the bottom of 1 of the 24-wells. I did a 500nm absorbance scan in a circular pattern (15×15 points) – to get data similar…
