Pericytes Contribute Basement Membrane Proteins in a Human Stem Cell-Derived Blood-Brain Barrier Model (Louis Widom’s 2024 MPS World Summit Poster)

In effort to understand the model, I have been studying simple diffusion experiments using rhodamine, a small molecule expected to experience little hindrance.  The model currently expects a small molecule the size of rhodamine to reach equilibrium within 1.5 hours.  In the following figure, the retentate is blue while the filtrate is green.   I…

Introduction This post is meant to introduce the new NPN-enabled slide-based devices produced by Ibidi. These devices feature an NPN membrane and microfluidic channels positioned perpendicular to one another formed by PSA and COP layers (Figure 1). Channels above and below the membrane allow for flow-based experiments including tangential flow filtration (TFF) such that biofluids…

With the installation of the new STED (STimulated Emission Depletion) system in the medical center, I have finally been able to work on imaging individual exosomes (that we know are exosomes because they are labeled for exosomal markers) that have been captured on our membranes. Now, I won’t go fully into the details of how…

Here are some images of deposited magnesium fluoride films on 50 nanonitride:              

As the title states, I partially etched some different material (Wafer 2238, 100 nm NPN) to create nanofeatures with blown out holes. With a thicker membrane, we can blow out the pores more (etch longer) without compromising the strength of the material (with regard to templating purposes). These membranes were fractured and coated with 6…

As we make larger and larger pores, we see that they are becoming more convoluted and have stopped looking like circles or ellipse. In order to try describing these shapes, they were given a “roundness” number which was calculated by taking the minor axis and dividing it by the major axis. However this often fails…