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Mark Lifson's Lab Rotation
The goal of this rotation was to create a device to test the electro-osmotic properties of the membrane bound silicon chips. To perform this task, it was necessary to attach two glass pipettes onto both sides of a chip. The method required that the protocol be repeatable and easy to perform. Although it appears to…
Electrolysis through TEM Chips
Continuing the project that Mark had been working on, I have made some progress towards electroosmosis. Specifically, I was able to observe electrolysis through an intact membrane. Hopefully I will be able to observe electroosmosis through the movement of microspheres or nanoparticles through the capillary tubes. I tried a few variations of Mark’s design using…
Air-Blood Barrier TEER Results
I finished 3 independent experiments of air-blood barrier co-cultures – these consist of bEnd3 endothelial cells on 1 side and RLE-6TN lung epithelial cells on the opposite side of the membranes. We didn’t expect these cell types to potentiate TEER in anyway (like we expected glial cells to tighten endothelial barrier function). However, our collaborators…
Endothelial cells on small square membranes
I designed new masks with different sized squares and slits in order to study how the aspect ratio of free-standing membranes would affect endothelial cells. In this 1st experiment, I seeded bEnd at 50000 cells/cm2 and let them grow for 4 days. I then stained the cells with Live/Dead and overlaid the phase channel in…
Reviewing membrane impact
To identify how much of an effect the membrane has on particular simulations, we originally came up with the idea of using a phenomenological effective diffusion to view the membrane impact (see this post). Here is the most up to date version of the figure, which was slotted for use in the simulated diffusion paper:…
Greg’s Proposal Exam Documents
Presentation: Madejski Proposal Presentation v3 Compressed Document: Madejski Silicon Nanomembrane Microsystem Proposal Final Passed in August 2015. Document was criticized heavily, oral presentation was thought to be good.