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Bradford Assay to Quantify Protein Retention During Desalting
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which show flux an order of magnitude greater than cellulose membranes. Our next step is to…
Physiological Facade: Fibronectin coating membranes in an attempt to improve the performance of Staphylococcus aureus propagation assays using the µSiM
Parallel with my efforts in the Osteomyelitis project to coat our membranes with polyurethane acrylate, I began working with the Gill lab on improving the performance of our uSiM platform as a propagation assay for Staphylococcus aureus. In the past we have gotten a lot of good data using this assay, but its performance has…
Wafer #251028-02 – 0.5 μm Microslit
Manufacturer’s Data Logged by: Ahmet Gurcan Membrane Type: MSSN 0.5 micron Microslit SKU: MSSN400.C-3LX.1-0.5E1 Lot Number: 251028-02 Quantity Received: 250 Manufacturer Test Report: Flow Data Avg in SLPM 4psi: 3.88 15psi: N/A Burst: 9.22 PSI
Gel Analysis
We have recently been discussing the best way to analyze the diffusion and SepCon separation gels. In the following figures, I present line scans of the gels. These appear to be a bit noisy and are somewhat misaligned due to variations along the length of the gel. Blue = Control, Green = Retentate, Red =…
Distributions of in-Situ formed Au Nanoparticles
Jirachai’s recent post on the formation of Au nanoparticles on the surface of the NPN is very exciting. For one, it is a very dense monolayer of particles. This self assembly mechanism is magnificent whereas if we tried to dry down a layer of particles, we would get a cake layer similar to what Josh…
Discoloration – Water soak
This week, I did a quick discoloration experiment based on Chris’s comment at lab meeting. He wondered if soaking the chips in water for a while would allow potential discoloration-causing contaminants to slowly leech out. I took 2 chips from wafer 622 and soaked them in ~ 300mL DIH20 overnight (with a couple of water…
