I set up diffusion experiments using the wafers that Tom was able to pass gold through in DI H2O using pressure. 5nm particles do not pass in 24 or 48 hours. If we have extra samples I will be testing 5nm particles in 10mM KCl.
I recently set up 24 hour diffusion separations with the BioRad protein standards. The following gel shows the separations using w415 position (-4,3) and w416 (-1,3). Different cutoffs were observed with the two membranes.
For a while now I’ve been working on developing and testing the practicality of using nanoporous membranes as filters for highly sensitive assays. Traditional filter-based assays are restricted in their speed by both the time it takes for a particle to find a pore as well as the time it takes for the particle to…
Introduction In the Glading Lab we study a rare disease called Cerebral Cavernous Malformations (CCMs). In this disease, brain capillaries develop greatly enlarged lumens and a disrupted blood-brain barrier. A genetic cause of the disease is the presence of a mutation in KRIT1 that causes loss of KRIT1 protein function in the cells. KRIT1 binds…
Theoretical molar flow is calculated for membranes with different porosities and is based on data extracted from TEM images using Mike’s program. Experimental data is calculated using air permeability test results. It is associated with porosity only through TEM data. Correlation between two is very good but some points do not agree. One of the…
Six wafers (385-390) were produced last week with the new Solaris 150 RTP system. Good news: We now have much better control/feedback compared to the RIT setup. There are two thermlecouples on the wafer tray that monitors the front and back portion of the wafer. The new unit allows us to independently modify the front/back/bottom…
An important result of last weeks experiment showed that Antimony (Sb) doped substrates had no pinhole defects; however, Phosphorous doped substrates from the same batch had many very small pinholes. This agrees with a previous experiment: Antimony wafers have shown no pinhole formation. I propose a model that aims to explain:1) Why we are not…