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Electrostatic Effects on Convective Flux of Nanoparticles: A Mathematica/Matlab Implementation of W.M. Deen’s Model
UPDATE: I have since ported the program into Matlab. If you open the following zip document(Archive) you can run Find_Sieving_Coefficient. You can select a results file and after ~5 minutes the expected sieving coefficient for the pore distribution is spat out. Using Wolfram Alpha’s Mathematica programming language, I’ve implemented an amalgamation of different mathematical models…
Updates on neutrophil-collagen migration experiments
In my previous blog, I alluded to the issues I was facing in achieving successful migration of neutrophils through the basement membrane into the collagen gels. I am successfully able to achieve transendothelial migration, but migration beyond the endothelial basement membrane is still not achievable. After a lot of thinking, I realized that this, in…
Retest: BSA and DDM diffusion with SC256 and 10mM salts
ByMike HYesterday I posted the results from an experiment testing diffusion of BSA and DDM with salts (10mM) across “treated” and “untreated” SC256 samples. The results posted yesterday showed relatively equal amounts of DDM diffusion in both the “treated” and “untreated” case. For the BSA diffusion however the results showed that a greater amount of BSA…
A Serendipitous Disaster of Interesting Proportions: High OD Staphylococcus aureus Pulldown on 0.5 µm Microporous Membranes
After titrating our pulldown volume to avoid pulling Staphylococcus aureus through our membranes, discussed here, we found that 10 µL withdrawals allow us to continue to use the pulldown modification without compromising our results by providing false positive transmissions. With newfound confidence in this pulldown modification, we took a step backwards to repeat a bacterial…
HUVEC Tight Junction and Cadherin Immunofluorescence
In my last post on HUVEC intercellular junctions, I just showed cadherin staining. I repeated this protocol but also tried immunofluorescence for ZO-1, the tight junction protein. These are HUVEC P7, 6 days post-seeding @ 50,000 cells/cm2. I looked at glass coverslips, pnc-Si and commercial PET transwells. Since background fluorescence was so high in my…
New laptop for the TECAN and DLS is finally hooked up
Over the past few days Josh and I installed windows 7, windows office suite, Malvern’s Zetasizer software (available online), Tecan’s I-control software (available in the 3-ring binder in the glass cabinet above the machines), and chrome onto the new(er) laptop in the basement. I copied the files on the old computer that seemed relevant –…

