From this updated post its evident that the O2 RTP treated samples start discoloring after ~10 days. Also, the O3 + O2 RTP treatment is probably better in preventing dicoloration than just O2 RTP. So amongst all the samples, the O3 + O2 RTP treated one starts discoloring at the last.
This week, Christine and I have been trying to figure out if we can measure transport in the Tecan with the transwell inserts remaining within the 24-well plates. Here is a schematic of our idea (with sodium fluorescein, Na-F, as the model fluorescent molecule for transport studies): The left image is a side view, the…
Previous Post We discussed in meeting having Maryna also calculating the permeabilities for this figure. Here is a figure with the experimental, theoretical permeabilities from Dave’s, Maryna’s, and my processing, and an average of the three theoretical permeabilities. I then took this average and made the following figure. I did omit w312, as the super…
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Tweaking simulation was not bringing it any closer to the experimental shown in the last post. This led me to try the experimental once more. I set up the same system and used 1:2 dilution of the standard protein mixture. I then homogenized the retentate and filtrate and ran 10 ul of each on a…
Earlier this week, Maryna and I did some AFM of her samples. She vapor-deposited parylene on the well-side of pnc-Si and was interested in whether/how well that polymer got through the pores. We got some really interesting-looking scans, but I’ll let her post those images. As controls, we looked at clean chips from the same…
In a previous post, we showed that both Cytochrome C and BSA at concentrations of ~1mg/mL through 20nm cutoff Sepcons with about the same filtrate concentration (~60% of the feed concentration). Yesterday (1/14/13) we repeated these experiments with 10 and 15nm cutoff Sepcons and demonstrated pretty clearly that either 10 or 15nm Sepcons can be used…