From this updated post its evident that the O2 RTP treated samples start discoloring after ~10 days. Also, the O3 + O2 RTP treatment is probably better in preventing dicoloration than just O2 RTP. So amongst all the samples, the O3 + O2 RTP treated one starts discoloring at the last.
Josh and I are taking a closer look at how the samples burst and what different factors can affect the strength of the membrane. We are specifically looking at minimum dimension, area, and perimeter. A wafer was designed that contained different sized samples. One of the three factors were held constant while the other two…
When the HUVECs were first brought up, the first test for me to perform was to see how they reacted to CMFDA and repeated fluorescent excitations. Based on the implications from previous research (summarized here), there is the possibility of harm or phototoxicity from the dye. Since HUVECs are more valuable and ‘fragile’ than either…
While I have been making nanoporous MgF2 for a while, it has become obvious that the strength of the material is handicapping our ability to generate data; as the membranes rupture we lose weeks of cell culture work. While we have generated some interesting Raman and cellular effects using the nanoporous MgF2 membranes, they are…
Last week, I looked at discoloration with different wafer numbers (639,670,672,683,685,702,703), pre- and post-rebuild. This experiment was done in the oven at 37C, in PBS and without RTP. Based on the starting color, w670 and w672 are the thinnest membranes. These discolored rather quickly (1 day). The only other membrane that discolored over 3…
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As a follow up to my previous post, I would like to present some preliminary data on the subject of endothelial cell alignment under shear stress with variable substrate stiffness. The flow set-up (Figure 1) allows for steady flow across the membrane on which the HUVECs are cultured. No Gel HUVEC Alignment Alignment of HUVECs…