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Rotation Project – Sergio Garcia
Introduction The Ehler-Danlos Syndromes are a group of thirteen heritable connective tissue disorders. These disorders are genetic, and usually involve a mutation in genes that code for components in the collagen matrix (1). Some of the main characteristics in patients with these disorders include tissue fragility, joint hypermobility, and skin hyperextensibility (1). Classical EDS (cEDS),…
BSA-Au results
w312 Number of Pores HistogramContributed Area Histogram 5nm BSA-Au is detected in the filtrate after 24 hours. 10nm BSA-Au is not. The 5nm registers around 10nm after conjugation and the 10nm is closer to 15nm. This selected membrane is not very porous, although it does have pores (as high as 42nm) that would appear to…
Pore tunability, consistency
In the last month, we’ve run several sets of 15 nm pnc-Si wafers over a range of RTP temperatures. Below is a chart that illustrates the current pore size control we have by tuning annealing temp. The 900 C, 950 C, and 1000 C samples were from wafers SC 231, SC 314, and SC 316,…
Anodic Bonding Setup at U of R
Previous Posts Anodic Bonding Theory, Anodic Bonding at RIT I’ve put together an anodic bonder setup here at the U of R and will be moving it inside the cleanroom shortly. I tried a couple of known combinations to see if the bonder was working properly. The general principle is to liberate sodium ions…
Simulating Protein Diffusion
I’ve been attempting to simulate protein sieving using the COMSOL model. This first figure shows the comparison of the simulated separations to the actual results as determined by gel densitometry. The simulated separations tend to lag behind the experimental data for smaller proteins. This is surprising since the simulation is faster than experimental results for…
Cell Labeling on Si
Below is an image of HUVEC grown on RTP treated W303 for 72 hours. The cells were seeded at high density and therefore reached confluence prior to day 3. The first row shows the membrane area of the sample; the membrane is not intact. The second row shows the edge of the sample. Live and…
