Adsorption Quantification II

This is probably not of general interest, but for the sake of full disclosure, I have spent considerable time working with our wafer supplier (Virginia Seiconductor) to resolve some issues regarding the surface quality of their wafers. It would have been much more productive to simply switch suppliers, but unfortunately, they are the only place…

The new oxide chips arrived this week, so I have materials for lots of experiments going forward. The current inventory stands as follows: ~100x NPN chips (mostly still in wafer form) 81x 20nm SiN + 200nm  + 10nm SiN cap 93x 20nm SiN + 200nm oxide Imaging of the membranes shows the expected images for…

I am doing a few more membrane transfers and trying to troubleshoot my delamination problems, and I wanted to run my protocol by you guys before I mess up any more chips. I was able to get about 3/7 transfers visibly very good without any wrinkles, but they all still delaminate, either during the fabrication…

To analyze the dimpled  Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples.  We’ve already shown that cells will grow on these membranes in our “Dimpled Sylgard Membrane Device” post and that our substrates are wetting in our “Degassing Experiments”…

Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and shown to work. At the lowest power setting on the Leica (~25%) fluorescence source, we were…