Adsorption Quantification II

Separations were performed at 2 different salt concentrations with equal volume filtrate and retentate (to avoid dilution problem). Separation time was 40hrs rather than the usual 24. The lower salt concentration run has a lower cutoff – which is just like what we see in the DNA separations. Remember though that proteins have different charges…

I set up separations with 100kD cutoff Microcon (cellulose) membranes and protein standards.  Here are the results: Due to shorter than normal gel, the myosin didn’t get resolved.  However we can see a separation that appears to be fairly sharp.  We have seen this in the past with cellulose.  The different salt concentrations don’t appear…

Purpose: Study the hemocompatibility of different substrates using platelet activation as the indicator.   Extraction of platelet rich plasma (PRP): PRP were prepared from whole blood following the standard protocol outlined by Abcam (http://www.abcam.com/protocols/isolation-of-human-platelets-from-whole-blood). To describe briefly, 9 mL whole blood was centrifuged at 200g for 20 minutes in a 15 mL Falcon tube to obtain…

Kelly & I deposited 100 & 300 nm thick SiO2 films on bare wafers with the P5000 PE-CVD tool at RIT last week.  The wafers were then annealed according to JP’s recipe overnight to stabilize the TEOS layer film stress.  The stress was measured using the Tencor P2 profilometer by scanning across it before depositing…