W613 Discoloration

This is my draft presentation for my CMSR presentation on December 4th. Link

Over the past week Andrea and I designed a new co-culture support structure for use with SiMPore’s 5.5 mm square membranes. The devices are built for use in standard 24-well plates and should prove useful to anyone wanting to do basic co-culture with these membranes going forward. The device is constructed of PDMS rings as…

Christine and I have been looking into whether the O-ring position affects transport through pnc-Si transwells.  The hypothesis was that the unstirred water layer under the Sepcon might be diminished by switching the O-ring from its normal position (“O-ring bottom”) at the bottom of the Sepcon to above pnc-Si (“O-ring top” or “pnc-Si bottom”).  This…

This week, Christine and I have been trying to figure out if we can measure transport in the Tecan with the transwell inserts remaining within the 24-well plates.  Here is a schematic of our idea (with sodium fluorescein, Na-F, as the model fluorescent molecule for transport studies): The left image is a side view, the…

A while ago, I posted some images of cells grown on commercial polyester (PET) transwells.  Here’s the post.  I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the bottom of the membrane.  I contacted Corning and they said that these dead cells were…

Last week, we created a series of wafers that were RTPed at four different temperatures between 900 C and 1000 C.  Below is a comparison of the 900 C (left) vs. 1000 C RTP (right) wafer. We see that a difference of 100 C shifts the cutoff ~10 nm.  The pores are also the roundest…