I did a small experiment to demonstrate that a carbonized membrane would be stable in a harsh alkaline solution, such as KOH. I soaked a carbonized membrane and untreated membrane in %5 KOH at 40 C for an hour in an oven. As you can see, the carbonized membrane is still in-tact and the untreated membrane has been completely stripped of Si.
There’s been a lot of production in the last month and we’re still sorting through the data, but here is a synopsis of some key findings. All films were deposited with the AJA tool. Film reproducibility Week-to-week variation in pore morphology has never been noted explicitly due to the rapidly changing conditions we place on…
I’ve taken fluorescence measurements with the fluorescence microscope on PEGylated and unPEGylated glass coverslips and SiN chips. Six coverslips and six chips were prepared. Samples were treated exactly the same, save for the fact that half the samples had PEG Silane in their treatment and half did not (though they were still bathed in toluene…
Yesterday I posted my results from a BSA diffusion experiment using “treated” and “untreated” SC269 samples. To ensure that the pores in SC269 are indeed open I took a step backwards and set up a diffusion experiment using small dye molecues; rhodamine and fluoroscein. Using Jess’ diffusion cell I set up a matrix to test…
Quick review of “Achieving more frequent and longer dialysis for the majority: wearable dialysis and implantable artificial kidney devices” by Fissell, Roy, and Davenport see Fissell(Roy)_2013_International_Kidney.pdf in the hemodialysis section of the library. First a little background: Two type of wearable devices: Peritoneal Dialysis (PD) and Hemodialysis (HD) In PD, dialysate is pumped into and…
This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells. These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days. Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but one survived this 7-day cell culture experiment. These cells were plated on the bottom on…
Cody Soule (MS student) developed/applied a protocol to functionalize the surface of a silicon oxide membrane. The process selectively attaches PNIPAM to the surface of the membrane, maintaining the pore characteristics unchanged. PNIPAM undergoes a configuration transition when going from 37C to RT, from collapsed to extended. It has been previously used to lift-off confluent…
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Chris –
I mis-spoke when I said the untreated membranes were stable in %5 KOH. It turns out that I diluted my last batch by 10X yesterday.
Terrific!
This could be very useful and it’s nice to have a coating in our pocket that protects the membranes. Hopefully more will be developed soon.
Are there spots on the untreated samples that did not etch? We those spots contaminated and visible before the etch?