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Updated: Continued Au separations with SiN-NP wafer #'s 1029 and 1030
Sarah and I did some more gold separations with 10, 20, 40, 50, 60, 80 and 100 nm Au particles. We used the “clean” water purchased from the medical center. Same as last time, we diluted the “as-bought” gold 3 parts to 1 and loaded each cell with 300 uL before pressurizing at 5 psi…
Karl's Air Permeability and Burst Pressure Raw Data
In this week’s meeting there was a request to have Maryna and I make our BP and AP data available online. While Maryna is at the Gordon Conference I thought I would upload what I have. The attached spreadsheet contains two tabs, one for AP and BP. Wafer number and position of each test sample…
Pressure Cell Update
This past week I ran multiple experiments using the pressure cell and sep-cons. I used membranes from W684 for the sep-cons since the burst pressure of these memebranes is high (around 3.65-10Psi). I added 100uL BSA to the Sep-Con and put 20uL 1xPBS on the backside. Before adding the NC to the sep-con, I assembled…
Conditioned DMEM – Discoloration
Motivated by Tom’s suggestion at lab meeting on Tuesday, I tried “conditioning” the DMEM+10%FBS before adding the chips. “Conditioning” just means that I allowed the DMEM+10%FBS to equilibrate in the incubator for an hour before adding the chips to test if a brief (~1 hour) exposure to very high pH causes discoloration. DMEM+10%FBS without the…
Desalting: Broken and Pinholed Membranes
Desalting of 4M NaCl in deionized water was performed using our membranes in the SepCon format. I ran the SepCons in the same as way as the previous study, using a low stir rate and floating the SepCons. Once again, a conductivity probe was used to measure the amount of salt in the dialysate. Transport…
Partial Etch RIE Blows out NPN Pores
As the title states, this post is about shifting an arbitrary pore distribution in an NPN layer. Why would we want to do this? We believe that the underlying pore distribution and shape influences the properties of many of our devices (my SERS sensor work included). Jim has coined the term ‘bowl-with-a-hole’ to describe the…