These chips were 2.9 mm TEM grid with a single 25 um window. 20 nm thick NPN is covered with 100 nm TEOS with a 2 um opening to expose ~1000 pores. Wafer number 2176.
Following are IV curve analyses for the above material as well as membranes treated with ALD alumina. These were measured in Vincent Tabard-Cossa’s lab.
So I think I have confirmed that PDMS is at least one of if not the major contributor to the contact angle problems in the UV-ozone system. Control image before any treatment: I then dried the chip and placed it in the UV-ozone system with two slides that each had two strips of PDMS. Since…
MATLAB updated the way that it creates GUI applications back in 2015, and it is increasingly becoming more functional. Over the years I have been here, we have had more than a few requests to use our pore processing software. I have sent it out, but it’s not as intuitive for some people to use…
Since the basic protein diffusion experiments went well, I’m moving up to something that more closely resembles an actual assay application. The basic idea is this: Biotin-conjugate FLAG antibody will be bound to Avidin acrylic beads in order to make them far too large to pass through the MP8NP50 membrane. Then, Bacterial Alkaline Phosphatase (bAP)…
Kilean and I are working on submitting a paper to Membranes on some of our electron tomography work. Here’s some of the work that we’ve done so far: Tomography Paper V2_KL
Introduction Read the manuscript here: Integrative Biology Publication Please refer to the linked manuscript for a detailed introduction. In short, we have been interested in characterizing the role of endothelial cell (EC) apicobasal polarity in inflammation. While usually reserved for the epithelium, ‘apicobasal polarity’ is a term describing a monolayer or cell with defined apical…
Two simple diffusion separations were performed before break using membranes from wafers 205 and 213 (locations unknown as these were salvaged/misplaced chips). I ran the samples I collected on a 10% SDS-PAGE gel today. It seems that the membranes were able to separate the standards at two different cutoffs. The missing band in the middle…