Hi All,
These are documents associated with my proposal: Silicon Nanomembranes for Rapid Liquid Biopsies from Whole Blood and Urine.
Proposal: Kilean_Lucas_PhD_Thesis_Proposal_Reduced File
Presentation: Proposal Presentation_03
Katia in Dr. Shestopalov’s Lab functionalized some pnc-Si windowless chips. The process she follows is shown in Figure 1. She provided chips at various stages of the process. “Grignard”, “Diazirine”, and “PEG”. After functionalization, the chips were wetted with 20 µL of 1:1 pbs then 40 µL of 5 mg/mL f-BSA was added. The…
I performed separations using C-dots from Hybrid Silica, a silica based particle with an internal fluorescent core. The particles are coated with a PEG layer of unknown length, but the idea would be that they’d be less affected by electrostatics than gold. I used two sizes. One is marked <10 the other <30, but I…
In order from left to right: 1) t=0, huvec on pncSi, in EGM (10% FBS) 2) t=24 hrs (cells reach confluence) 3) DIC after staining 4) FITC (live cells) 5) Rhodamine (dead cells) Its good to see the cells reach confluence and the membranes intact at the same time! There is a decrease in number…
Lately, Anant and I have been doing our discoloration experiments under purposely harsh conditions so we don’t have to wait for multiple days for experiments to finish. We have lots of evidence that the 3D format slows down discoloration (post1, post2, post3). This experiment was to test how long samples will last in 3D format…
Conventional tissue culture substrates do not accurately emulate in vivo surface conditions. This project involves the development and characterization of a polydimethylsiloxane (PDMS) substrate for cell culture. PDMS is an important biomaterial due to its optical transparency, biocompatibility, and variable elastic modulus. ADSCs and HUVECs were used to analyze spreading and proliferation on PDMS-based substrates of…
The AIM of this experiment was to see the effect of surfaces modified with PEGs (Polyethylene glycol) to protein adsorption. The PEG that I used for the current study is MS-PEG8 (Methyl-PEG8-NHS) which is Amine reactive. Method: For the current experiment I used planar silicon chips which have ~ 142 nm of thermally grown oxide….