The basics of this device are… Blood flows through a filter made of zeolite containing nanofibers. While the blood flows transversely through the mesh, creatinine can diffuse through the mesh where zeolites stand by to grab them via size selecting capture. Their experiments were done with 190 µM creatinine solution. MW of creatinine is 113.1179…
Josh Miller came up with the idea of filling individual damaged membrane channels with PDMS so the rest of the chip could be salvaged. We worked on it this week, mostly Josh, and we developed a process using strips of silicone laid on the top (flat) side of the damaged channel and PDMS injected onto…
On Wednesday Feb. 12 and Wednesday Feb. 19, limited exposure dialysis was performed on rats. In the first experiment, blood was pumped with the peristaltic blood pump through a two-chip dialysis device in an acrylic fixture. The membranes were non-porous silicon nitride so no dialysate was included in the experiment. Blood was successfully pumped through…
The overall aim of the small animal dialysis is to evaluate the dialysis membranes and compare their urea clearance to commercial materials. The specific aim of this study was to go through the process of ordering and receiving rats, setting up the experiments and working out the connections between the catheters and the test devices….
Helpful Hint: H<sub>2</sub>O typed as text will yield H2O visually, and E = mc<sup>2</sup>O typed as text will yield E = mc2. Of course this affects line height, so choose your poison, I guess.
Earlier this year an MIT/Harvard group generated and transplanted a rat kidney and published in Nature Medicine Technical Report. “Regeneration and experimental orthotopic transplantation of a bioengineered kidney” Jeremy J Song, Jacques P Guyette, Sarah E Gilpin, Gabriel Gonzalez, Joseph P Vacanti, & Harald C Ott Center for Regenerative Medicine, Massachusetts General Hospital and Harvard…
Images of the nanoporous nitride wafer. The same wafer that Josh declared as having 30% porosity with an average pore size of 80 nm (or something similar). Josh put images and such on basecamp for those who have access. These numbers are for a TEM grid from near the outside edge of the wafer, where…
Sprague-Dawley rats will be purchased from Harlan. These pre-manipulated animals will have been catheterized prior to shipment. We will feed them an adenine rich diet to induce uremia, high levels of urea in the blood. Once uremic, a rat will be connected via blunted needles to the dialysis system. Heparin, or other substances, may be…
In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two pnc-Si chips mounted in teflon block with blood flowing between the chips and dialysate flowing…
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic