I was given three groups of chips to SEM. The first group had undergone the Diazerine and PEG1000 treatment followed by incubation with either PBS, IgG, or BSA. The second ‘group’ was a chip that had been treated with diazerine and PEG1000 but had not been incubated. The last group was a chip which had…
Hemodialysis chips are not surviving the priming process and the 4 hour dialysis treatment. We want to verify that the membranes are being weakened and also to determine if coating with oxide will ameliorate the problem. Using single slot chips from wafer 1153 I performed burst pressure tests on three groups of chips. 1. Bare chips…
We are continuing to work with the Shestopalov lab on surface modifiation of hemodialysis filters to prevent adhesion and activation. Jim recently presented our status and strategy (PDF) to the Technical Development Fund and was awarded the balance of the award, so we may continue our work. Our goal is to develop hybrid hemodialysis devices to enable…
I went to Kidney Week in Philly, Nov 13-15. American Society of Nephrology. I spent most of my time in the Bioengineering talks and posters. Here are some highlights. I put the Abstracts pdf on the cloud. Recellularization of Vascular and Tubular Compartments of Rat Kidney Scaffolds with Embryonic Stem Cells Basically, they perfused…
Discussion: The BSA may have not been fresh enough to give good data, the difference between background and signal are pretty low. The Rhodamine IgG gave better signals but the PEG4 treated SiN is between the untreated SiN and bare pnc-Si. The latter having a lower signal. Here are the details. The fluorescent BSA was…
BOTTOM LINE: We had great results from the small number of nanoporous membranes used to clear urea from Sprague-Dawley rats. We are clearing Urea better than commercial materials. (see fig) Recap of previous work: First we leaned how to set up the dialysis experiments using Sprague-Dawley rats. We made some rats uremic to elevate the…
This is the method Brian McIntyre developed for the recent URNano Microfluidics Workshop. Based on a polyaddition-based mechanism based on introducing amine groups onto the surface of one material and epoxy groups on another material and enabling the amine–epoxide polyaddition reaction at their closely contacted solid-to-solid interface to establish a covalent and permanent bonding presented in…
The Bowman’s Capsule contains the Glomerulus, these are both part of a nephron of which the kidney has a gazillion. (The real estimate is the each kidney contains a million nephrons) Here are some pics to orient you. These give some idea why the Fissel group uses slits. The glomeruli are the main filtration device…
We performed four hours of sham dialysis, using two non-porous nitride membranes. The set-up is described in an earlier post. Blood samples were taken when the animal was received, after one week of the adenine containing diet, then at 1 hour intervals during the dialysis. The data shows that the urea level, which was increased over…
In order to test the efficacy of any dialysis treatment, we need induce uremia in our animal model. We choose to forgo the surgical approach of performing a 5/6 nephrectomy via ligation for the simpler diet based approach. Feeding the animals a diet containing adenine is meant to raise the level of urea in the…