Super useful conversions between volumes and lengths
1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
Thanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published in Communcations Biology and will soon be located at this DOI. What follows are key…
To keep the NRG up-to-date on the goings-on in our collaboration with the University of Ottawa, we will plan to share our progress slide decks from the CRePE-ssNP group meetings that happen every two months. Periodically, we’ll also update the NRG on overall progress of the project including the work done in the two Ottawa…
R61; 12 month Progress (Rochester) Small Trans-Agency Blood-Brain Interface Program Investigator Meeting – Agenda_FINAL
One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289 where he showed that stimulation of ECs with TNFα from the abluminal chamber caused an…
Introduction In addition to in situ measurements of small molecule permeability (Parts 1, 2 and 3), we wish to enable an assessment of permeability by sampling from the abluminal chamber of the µSiM in a manner similar to what is done with Transwells®-style devices. To begin this, we need to review the methods used with…
This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position beneath the membrane. In Part 2 we validated this approach experimentally on the Andor Dragonfly…
PDF with all slides: R61 HL154249-01(6 Month Progress)
This is the second installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position beneath the membrane. Because our preference is to use the trench down configuration in the…
NOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on application in the R61 project. Small molecule diffusion from the well to the channel side…