w565 is permeable to rhodamine. I tested two chips using 100uM rhodamine using a time step of .5 minutes. Click on pictures to see animations. position (1,-1) – While trying to do this first sample, MATLAB crashed and I was forced to take the movie about 7 minutes after the start. There is also a…
Previously we have worried about the validity of our adsorption data considering we have used it to test both sides of anisotropic membranes that have cutoffs below the size of albumin. Today we came up with the idea that albumin can be used as a probe for adsorption, not as simply a model protein in…
UPDATE: Teflon data now include Original Post: Refer back to Part I, and Part II This weekend I ran a bunch of gels to flesh out the adsorption data a bit more. I tested MicroCons, NanoSeps, and teflon and reran the initial Pnc-Si gel to get rid of negative results due to uneven staining. To…
Hybrid silica studies the size of their particles by fluorescence correlation spectroscopy. I used our dynamic light scattering instrument to see what sizes it would get from these particles. Their 45nm particles: Avg center of peak = 46.2 nm Avg width = 15.6 Their 22nm particles: Avg peak center = 27.8nm Avg width = 7.5nm…
I’ve updated the figure from this post to add new data from oxidized and pre-washed glass samples. We now have possession of teflon material, which I will be testing this week.
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done. This data has all been obtained using the more vigorous washing step. All densitometry performed…
In our previous adsorption study, all of the surfaces seemed to adsorb a similar amount of protein. We thought this might be due to ineffective washing. In this study I tried a better washing technique: Incubate polymer membranes, pnc-Si chips, and glass in 1mg/mL BSA for 30min Rinse by vigorously swishing in 1500mL PBS for…
Glass chips and pnc-Si membranes were incubated in 1mg/mL FITC labeled BSA for 30min. They were vigorously washed in a large beaker of PBS. Chips were placed on a slide and imaged with fluorescence scope. For this study, it seems that the fluid surrounding the chip was much brighter than the chip itself. The following…
I set out to do an in depth study of adsorption to various surfaces using the sample buffer method. Midway through staining the multiple gels I noticed there was a problem. It looked as though glass and polystyrene adsorbed about the same amount of BSA as did pnc-Si. Details of the setup follow. Preparation: Using…
In the previous adsorption trials I compared SepCons to MicroCons and NanoSeps. The SepCons appeared to have higher adsorption, which we thought may have been a result of the plastic used in the SepCon. I then removed membranes from MicroCons and NanoSeps and compared to pnc-Si membranes in the same manner as outlined in the…