The following diagram lays out each separate setup that was tested using different combinations of Geltrex and substrates. Each trial has a different reasoning behind why it was done. The purpose of these trials was to investigate the effects of Gelrex gel and PCTE membrane inserts on tube formation in Bend.3 cells. First, we showed…
I have been working on the in-situ heating experiments on the free-standing membranes in the past few weeks. After quit a few times attempts I finally have some good videos made from the TEM images that were taken during the in-situ heating process. First I’ll introduce the background of these two videos including sample info,…
After a false start with non-microporous TEOS chips, I quickly did an image comparison with truly porous chips using polystyrene beads. The TEOS and Silicon Nitride chips were both fabricated in the single-well CytoVu format (single 2 mm window, 5.4mm die size). The TEOS chips allows water and media with serum to much more easily…
The initial measurements with multiple tests tubes containing either Serum or PBS failed to demonstrate evaporation and in fact the weights of the collection tubes increased after sitting in the refrigerator overnight. Perhaps the tape collected water? At any rate (<– trite expression), I redid the experiment with a single tube of serum. The tube…
Two experiments were performed to explore application of SiMPore filters within INT’s microfluidic cartridges to allow the capability for pathogen type specificity in nucleic acid purification. 1) The first experiment tested the efficiency at which nucleic acids could pass through the membranes. T4 polynucleotide kinase was used to 32-P radiolabel two different sized PCR products:…
Summary of Results Membrane Pore Diameter Thickness Porosity Permeability (cc/cm^2*min*bar) SiN 3.2-4.0 um 0.12 um 22.1%-35.3% 30000 PCTE 2.4-3.0 um 9 um 11.9%-16.1% 1652 SiN Experimental Design Permeability of the 3 um pore Si Nitride membranes was assessed using the pictured apparatus: Fluid (ultrapure water) was pumped at a constant rate into a pipet whereupon…
After discussions with the gentleman in charge of surface functionalization at INT last week, Jim suggested that I attempt to pretreat our membranes with 0.1% SDS before a filtration. The hope being that some surfactant left on the surface of the membrane would be enough to facilitate water passage through the membrane without wetting the…
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This summer I’ll be working on preliminary development of technology that might lead to SiMPore drug discovery assays. The goal is to use SiMPore membranes to create much more efficient and generalized assays, which represents a big opportunity for the company. However, several challenges need to be addressed in order to make such a device…
Can someone help explain this e-mail? Hey Jim – Just got a call from the locksmith who had a report from security that someone in your lab is tampering with the lock on your lab door…by doing what they’re doing, they are able to get into your lab without a key (or swiping). It’s been…