I made a lot of 6 devices for Vaccinex. We attempted to seed human endothelial cells on the membranes, flowing at 20 uL/min, first coating the surface of the membrane with an adhesion molecule at 20 uL/minute. At the same time, the same cell line was grown in a 24 well TEER z-scope tool to…
Quick review of “Achieving more frequent and longer dialysis for the majority: wearable dialysis and implantable artificial kidney devices” by Fissell, Roy, and Davenport see Fissell(Roy)_2013_International_Kidney.pdf in the hemodialysis section of the library. First a little background: Two type of wearable devices: Peritoneal Dialysis (PD) and Hemodialysis (HD) In PD, dialysate is pumped into and…
My past devices that utilize a membrane to separate particles have been haunted by mysterious pieces of junk. The other day I took a moment to inspect the holes that are punched using a sharpened 16ga needle in order to mate capillaries. 10x brightfield image of a punched hole, viewed horizontally …
Wafer # 4369 (PFI 02) was fabricated with pore sizes ranging from ~550 – 600 nm. Below are two SEM images of the pores, followed by two STEM images. The pores are all open and very well defined. The only defect I could find was a slight stitching offset in the patterning as seen the…
Are you wondering why Dean has yet to go beyond 30% BSA in his clearance studies? Here is why … APP-04_Viscosity-of-BSA-in-PBS.pdf
We have often encountered cytochome C dimers as part of our work. For example in this figure from Jess that we often refer to in our hemodialysis work. It turns out that Cyto C polymerization is a well known phenomenon. The dimers are very tough and survive the denaturating conditions in Jess’ gel. Here is…
Hello everyone! The new FEI Osiris TEM system has been finally setup recently. With the new purchased Gatan single tilt heating holder 628, we were managed to do an in-situ heating experiment with the Osiris system last week. We used the same sample OSO201510, which was used in Harvard, to test this heating holder as…
Power Point for my Senior Design https://docs.google.com/file/d/0B1AH05-mf_tQLTdSMHRWX0NjNGc/edit?usp=sharing
This is my first attempt of depositing PEG using vapor deposition methods, in a controlled fashion. The stepwise procedure: Josh was extremely kind by providing me with a diced pncSi wafer [windowless]. He taught me how to do the buffered oxide etching to remove the native oxide from the surface. This step is just to…
A control was done to observe cell proliferation on a TPM coated cover glass. Images were taken at 10x after 4 days. TPM coated cover glass Polystyrene petri dish We are having some success with the seeding of bend.3 cells onto microarrays sterilized with UV exposure. These were allowed to grow for 4 days. Images were taken…