A new user in URnano has used the “old” susceptor for a ~270 C (I might have the exact number wrong, but it is relatively low temp) to heat In on GaAs. I don’t think there was ever a good reason for using the RTP for this process, but what is done is done. I…
Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and DI or PBS. We are testing for what will make the most optimal cell culture…
I ran a 30 hour urea clearance with 100% serum through the single slot dialysis device. The now standard protocol was run. The single slot chip was annealed in the RTP for robustness then clamped into the acrylic/PDMS flow fixture. Ethanol was pumped through the chip for 1 hour, then degassed PBS was pumped for…
What process flows have I used to create my devices? Standard Cut out gaskets (10 min) Embed Electrode (face down) (3 hrs) Ozone Bottom, Interstitial, Filter and Top channels together (1 hrs, cure overnight) Ozone Bottom Electrode (1 hr, cure overnight) Ozone Top Electrode + Feet (1 hr, cure overnight) Weaknesses are that there are…
Dr. Webb is very interested in the thin nanomembranes that our group experiments with. To date, he has been trying to get cells to grow on SiN microporous material and image them with a few different types of microscopy (Phase, Confocal, Scanning Conductance Ion Microscopy). The cells have not grown well natively on the nitride…
The Cambridge Microsystems Savannah 200 atomic layer deposition (ALD) system uses a two-step chemical process that alternates pulses of water vapor and trimethylaluminum (TMA) to build a layer of Al2O3 on a silicon substate. Because of the two-step nature of the process, the film is forced to grow by successive monolayers, each with a thickness…
Karl and I have been working to measure separations using SEPCONs with particles ranging in size from 20 nm to 1 µm. We started out naively enough by slightly diluting some flourospheres we found in the refrigerator, putting them in the TECAN’s nanoquant plate and pressing run. I know. Ha ha. The first point we should…
I created some quick open air devices to test the viability of different adhesion molecules. The basal well has a volume around 150 uL, and the apical well has a volume around 15 uL. I made 6 devices in this format. On 5/14/13, Alan and I filled the basal chamber with PBS and then…
In my previous post talking about the contact angle measurement of 3-mer coated PEG, there was a lack of adequate controls, and also an unanswered issue of contamination of samples during storage. I would now like to refine the study in more -controlled fashion, and without reusing the old chips. In addition, I am also…
I found this article about a macro-microfluidic interconnect that I want to try out at some point. We might even be able to seal tube-block interface with PDMS for a tighter seal. http://blogs.rsc.org/chipsandtips/2006/11/13/a-simple-interface-between-a-pdms-microfluidic-device-and-a-syringe/ A simple interface between a PDMS microfluidic device and a syringe 13 Nov 2006 By Francesca Burgoyne. Jeonggi Seo and Meng…