Wafers 525 & 527 have a 1000 Å dry thermal oxide mask with all three films on the frontside sputtered. Both these wafers had a high density of pinholes. The pores are also irregular in shape and seem to have material “hanging” off the edges of the pores. Could this be a consequence of depositing…
W502 and 504 were tested for burst pressure and air permeability. Only one sample from W502 was tested for burst pressure (the other broke while setting up and I wanted to conserve samples) with a burst pressure of 4.46 psi. The burst pressures for W504 ranged from 2.06 to 6.57 (n=3). Air permeability is shown…
HUVEC (P8) growth was monitored over 5 days on different substrates (glass coverslips, tissue culture treated plastic and pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi in two different trials were observed to be pretty much the same. Decrease in the growth…
A second fibroblast (L1 3T3, P21) growth test was conducted on RTP treated samples from W510, tissue culture plastic (polystyrene), and glass cover slips. Fibroblasts were cultured in DMEM with media changed every 48 hours. Cells were imaged using the live/dead assay. Fibroblasts divided every 29.9, 35, and 37.7 hours when grown on pnc-Si, glass,…
Last week’s production yielded two wafers (502 & 504) with defect-free membranes. 506 had a high pinhole density. These wafers have a TEOS mask and were annealed twice; the first anneals did not produce pores so they were annealed again at 1000 C. From these results, it seems that the first anneal “seeds” pore formation…
I set out to do an in depth study of adsorption to various surfaces using the sample buffer method. Midway through staining the multiple gels I noticed there was a problem. It looked as though glass and polystyrene adsorbed about the same amount of BSA as did pnc-Si. Details of the setup follow. Preparation: Using…
I recently tested the burst pressures of samples from W510, 511, and 513. None of the membranes are pinhole free. Silicon thickness varies (15 nm, 10 nm, 20 nm). See Dave’s post for complete fabrication information. W513 has noticeably higher burst pressures compared to W510 and 511. Overall W510 is in the worse shape overall,…
In the previous adsorption trials I compared SepCons to MicroCons and NanoSeps. The SepCons appeared to have higher adsorption, which we thought may have been a result of the plastic used in the SepCon. I then removed membranes from MicroCons and NanoSeps and compared to pnc-Si membranes in the same manner as outlined in the…
This is the protocol I use to measure band intensities. At the end of the explanation I will post and review my recent results concerning silver stain densitometry. First load a digitized gel into ImageJ Select Image>Type>8 bit to make it black and white. Select Invert to make the bands have positive intensities. Go to…
I recently attempted a fibroblast (L1 3T3 cell line) growth test. The cells were seeded at too high of a density, so growth curves can’t be produced. However I checked cell viability and pnc-Si discoloration. As expected RTP treated samples discolored later than treated samples. Samples with cells seemed to discolor sooner than samples without…