μSiM

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in situ Permeability Analytical Solution Summary – Unhindered vs Hindered D
ByMolly McCloskey October 20, 2021January 18, 2023

Introduction Pages regarding our in situ permeability assay development can be found in these links: Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach) Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 2: Experimental Validation) Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 3: Application to Cell…

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Competitive ELISA assay to measure the HA shedding from HUVEC monolayer
ByChloe Chen September 28, 2021

Competitive ELISA assay to measure the HA shedding from HUVEC monolayer Introduction The endothelial glycocalyx is a dense layer of proteoglycans, glycoproteins, and glycosaminoglycans (GAGs) that helps maintain the integrity of the endothelial cell (EC) barrier.1 The main GAGs are heparan sulfate (HS), chondroitin sulfate, and hyaluronic acid (HA), among which HS and HA are…

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COMSOL simulation on sampling method to measure membrane permeability
ByHoward Su September 26, 2021September 28, 2021

Background We at Michigan have been aiming to perform sequential small volume sampling coupled with our ultrasensitive digital immunoassay to provide longitudinal data from the uSIM. We have encountered repeatability problems as well as low molecule abundancy with shortly incubated samples collected from the bottom microfluidic channel. Therefore, COMSOL simulation work has been done to…

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Sampling Method Permeability Assay Optimization
ByMolly McCloskey September 26, 2021

Motivation We have discussed the optimization of our in situ permeability assay at length. This assay relies on confocal microscopy, which may not be accessible to all users of the µSiM. Therefore, we developed an alternative permeability assay in the µSiM that is an endpoint, sampling assay. The protocol for this assay can be found…

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Thinkin’ Secretion in the µSiM
ByJim September 10, 2021September 28, 2021

One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289 where he showed that stimulation of ECs with TNFα from the abluminal chamber caused an…

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Assessing Neutrophil Activation inside a Flow Cell Device with Flow Cytometry
ByDan Ahmad August 27, 2021

Introduction As part of our continuing evaluation of the flow cell developed by our collaborators at RIT, our next goal is to demonstrate that neutrophils remain inactivated when flown through a first generation device (Figure 1). For this, we studied neutrophil activation as a function of fluid flow induced shear stress based on the physical…

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Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 4: Sampling)**
ByJim August 10, 2021September 28, 2021

Introduction In addition to in situ measurements of small molecule permeability (Parts 1, 2 and 3), we wish to enable an assessment of permeability by sampling from the abluminal chamber of the µSiM in a manner similar to what is done with Transwells®-style devices. To begin this, we need to review the methods used with…

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Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 3: Application to Cell Barriers)*
ByJim June 28, 2021November 1, 2021

This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position beneath the membrane. In Part 2 we validated this approach experimentally on the Andor Dragonfly…

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10dynes/cm^2 Aline bottom channel
ByJulie Kuebel June 22, 2021

AlineFlowBottomChannel

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10 dynes/cm^2 bottom channel Aline
ByJulie Kuebel May 26, 2021June 1, 2021

AlineFlowBottomChannelSup AlineFlowBottomChannel

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