We have seen before that silanization slows down discoloration and hence prevents membrane degradation. In Sepcon, the sample should last over a week. Further, it was necessary to check whether silanized surface supports normal cell growth. Therefore, comparison of cell adhesion on aminosilanized surface is made to control surface (non silanized). I took samples from…
Due to our desire to improve membrane stability in cell culture, different methods of treatment have been mentioned to try, including carbonization and silanization. W606 was treated with UV/ozone at 150C for 20 minutes, followed by silanization performed by some of Chris’ colleagues. I then ran a basic discoloration test in DMEM +10% FBS (the…
Previously W514 was tested with different RTP conditions (varying time at 800C, all in Ar); the results were difficult to interpret as the cells were confluent (in some cases it was hard to see membrane breakage). We therefore decided to run a similar test again, with a slight change in O2 addition during RTP. O2…
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs. HUVEC spreading on pncSi HUVEC spreading on glass
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant improvement from sample lifetime of 15nm thick pnc-Si. To see if RTP treatment helped the…
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on glass 1 Fibroblast spreading on glass 2 Time lapse movies for fibroblasts spreading on pncSi…
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time lapse movies can be seen from the following links: huvec_glass_sprd huvec_membrane_sprd fibroblast_glass_sprd fibroblast_membrane_sprd Somehow its…
Four different post-production treatments with the objective of improving membrane stability in culture were run last week on W615, a pinholed wafer laden with the circular features we have been seeing recently. I ran four different conditions for cell culture: no treatment (n=3) UV/ozone for 5 minutes at room temperature (n=4) RTP in Ar for…
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no significant difference in diffusion between the samples after they are RTPed, as expected. Now, Henry…